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目的:通过观察大肠腺瘤、腺癌和正常大肠组织中的细胞凋亡、细胞增生及其调控基因Bcl-2,Bcl-Xl,C-myc和核因子-κB的表达状态,探讨核因子-κB在大肠癌发病过程中的作用。 方法:利用DNA缺口末端标记技术和NF-κB,Bcl-2,Bcl-Xl,C-myc,PCNA蛋白免疫组化染色,原位检测30例大肠腺瘤和30例大肠腺癌中凋亡细胞、细胞增生和NF-κB,Bcl-2,Bcl-Xl,C-myc阳性表达细胞的密度和分布,以10例正常大肠组织作对照。 结果:NF-κB,Bcl-2,Bcl-Xl,C-myc蛋白的表达率和表达强度腺癌和腺瘤高于正常组织(70.9±7.2 vs 54.0±4.5 vs 30.6±2.4;78.2±8.3 vs 55.6±6.5 vs 11.0±1.9;77.3±6.5 vs 56.4±4.7vs 9.3±1.1;70.3±6.7 vs 50.1±4.2 vs 14.2±1.7,P<0.01),腺癌高于腺瘤(70.9±7.2 vs 54.0±4.5;78.2±8.3 vs 55.6±6.5;77.3±6.5 vs 56.4±4.7;70.3±6.7 vs 50.1±4.2,P<0.01)。腺瘤和腺癌中的凋亡细胞指数均高于正常组织(105.9±6.1 vs31.5±3.7 vs 10.0±1.5,P<0.01),腺瘤高于腺癌(105.9±6.1vs 31.5±3.7,P<0.01)。腺癌和腺瘤中的细胞增生高于正常组织(75.4±5.6 vs 50.7±4.9 vs 29.6±3.0,P<0.01);腺癌高于腺瘤(75.4±5.6 vs 50.7±4.9,P<0.01)。 结论:NF-κB在大肠肿瘤中促进细胞增生、抑制细胞凋亡,在其发病过程
OBJECTIVE: To investigate the expression of Bcl-2, Bcl-Xl, C-myc and nuclear factor-kappaB in colorectal adenoma, adenocarcinoma and normal colorectal tissue, and to explore the nuclear factor- The role of κB in the pathogenesis of colorectal cancer. METHODS: Apoptotic cells were detected in situ in 30 cases of colorectal adenomas and 30 cases of colorectal adenocarcinomas by DNA nick end labeling and immunohistochemical staining for NF-kB, Bcl-2, Bcl-Xl, C-myc, and PCNA proteins. Cell proliferation and cell density and distribution of NF-κB, Bcl-2, Bcl-Xl, and C-myc positive cells were compared with 10 normal colon tissues. Results: The expression and expression intensity of NF-κB, Bcl-2, Bcl-Xl and C-myc protein were higher in adenocarcinoma and adenoma than in normal tissues (70.9±7.2 vs 54.0±4.5 vs 30.6±2.4; 78.2±8.3 vs. 55.6±6.5 vs 11.0±1.9; 77.3±6.5 vs 56.4±4.7 vs 9.3±1.1; 70.3±6.7 vs 50.1±4.2 vs 14.2±1.7, P<0.01). Adenocarcinoma was higher than adenoma (70.9±7.2 vs 54.0± 4.5; 78.2±8.3 vs 55.6±6.5; 77.3±6.5 vs 56.4±4.7; 70.3±6.7 vs 50.1±4.2, P<0.01). The apoptotic cell index in adenomas and adenocarcinomas was higher than that in normal tissues (105.9±6.1 vs31.5±3.7 vs 10.0±1.5, P<0.01), and adenomas were higher than adenocarcinomas (105.9±6.1 vs 31.5±3.7, P<0.01). The cell proliferation in adenocarcinoma and adenoma was higher than that in normal tissue (75.4±5.6 vs 50.7±4.9 vs 29.6±3.0, P<0.01); adenocarcinoma was higher than adenoma (75.4±5.6 vs 50.7±4.9, P<0.01). . Conclusion: NF-κB promotes cell proliferation and inhibits apoptosis in colorectal tumors during its onset