论文部分内容阅读
在十六烷基三甲基溴化铵（ＣＴＡＢ）／异辛烷－正戊醇反相胶束中，研究了含水量（Ｗ０）和表面活性剂对辣根过氧化物酶（ＨＲＰ）活力的影响机制。在测定不同含水量（Ｗ０）和ＣＴＡＢ不同浓度下的ＵＶ－Ｖｉｓ光谱（即Ｓｏｒｅｔ吸收光谱）及活力的变化的基础上，发现含水量不同时，反相胶束主要通过影响ＨＲＰ的活性中心而影响酶的活力，但ＣＴＡＢ对酶活性中心没有明显影响。此外通过反相胶束与水相中的ＨＲＰ与Ｈ２Ｏ２复合物的Ｓｏｒｅｔ吸收光谱的变化，对其稳定性进行了对比研究。结果表明：ＨＲＰ与Ｈ２Ｏ２（摩尔比为１∶２０或１∶１００时）形成的ＨＲＰ－Ｉ和ＨＲＰ－ＩＩ在反相胶束体系中消失速度很快，且ＨＲＰ的Ｓｏｒｅｔ光谱变化与水相中不同。这说明了在反相胶束中ＨＲＰ与Ｈ２Ｏ２复合物相当不稳定。所有这些现象部分地解释了反相胶束中酶活力下降的原因。
In cetyltrimethylammonium bromide (CTAB) / isooctane-n-pentanol reversed micelles, the effects of water content (W0) and surfactant on horseradish peroxidase (HRP) The impact mechanism. Based on the determination of UV-Vis spectra (ie, Soret absorption spectra) and the changes of vitality under different water contents (W0) and CTAB concentrations, it was found that reverse micelles mainly affect the activity center of HRP when the water content is different Affect the enzyme activity, but CTAB has no significant effect on the enzyme activity center. In addition, the stability of HRP and H2O2 complexes in reversed phase micelles and water phase was studied by comparing their Soret absorption spectra. The results showed that the HRP-I and HRP-II formed by HRP-H2O2 (molar ratio of 1:20 or 1: 100) rapidly disappeared in the reversed-phase micellar system, and the Soret spectra of HRP changed with that of the aqueous phase different. This shows that HRP and H2O2 complexes are rather unstable in reversed micelles. All of these phenomena explain in part the reason for the decrease in enzyme activity in reverse micelles.