红瑞木茎段组织培养技术研究(英文)

• 作 者: 张杨 李周岐
• 机 构: 西北农林科技大学园艺学院,西北农林科技大学林学院,
• 出 处: 西北林学院学报
• 发表日期: 2010年06期
• 关键词: 红瑞木 组织培养 生长调节剂 增殖系数 生根苗 shoot sterile 生根诱导 auxin 西北林学院学报

以红瑞木茎段为外植体,研究了其组织培养技术。结果表明:适合红瑞木芽启动培养的培养基为MS+BA0.5 mg·L~(-1)+IBA0.5 mg·L~(-1),诱导率可达93%;增殖培养基以MS+BA0.5mg·L~(-1)+IBA0.3 mg·L~(-1)为最佳,增殖系数可达4.15;生根诱导的最适培养基为1/4MS+IBA0.05 mg·L~(-1),生根率可达90%;生根苗在温室条件下成活率可达80%以上。

In this study, the tissue culture technique was studied with stem segments of Osmanthus fragrans as explants. The results showed that the optimum medium for the initial culture of Rhodiola sachalinensis was MS + BA 0.5 mg · L -1 and IBA 0.5 mg · L -1, with the induction rate of 93% MS + BA0.5mg · L -1 and IBA0.3 mg · L -1 were the best, and the multiplication coefficient was 4.15. The optimal medium for rooting was 1 / 4MS + IBA 0.05 mg · L -1, the rooting rate can reach 90%. The survival rate of rooting seedlings in greenhouse can reach more than 80%.