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为确认浙江省首例疑似人禽流感病例,进行病原学分析,对患者气管吸出物进行核酸RT-PCR、荧光定量RT-PCR检测以及病毒分离,并对患者血清进行HI抗体测定。结果表明:患者气管吸出物H5N1亚型和A型流感病毒特异核酸均呈阳性,分离到禽流感病毒A/Zhejiang/16/06(H5N1)株;双份血清中禽流感病毒(H5N1)HI抗体滴度分别为1:320和1:640,从病原学和血清学上证实为人禽流感病例。分离毒株测序结果显示,A/Zhejiang/16/06(H5N1)株在HA裂解位点为多个碱性氨基酸,符合高致病性禽流感病毒特征;该毒株的HA、NA、PB2、NP、M和NS基因序列均为禽源,与2005年我国福建、安徽等地禽流感病毒分离株高度同源,而与越南、泰国以及香港1997年分离到的禽流感病毒株之间存在明显差异。
In order to confirm the first case of suspected human bird flu in Zhejiang Province, etiological analysis was carried out. The patients’ tracheal aspirates were analyzed by RT-PCR, fluorescence quantitative RT-PCR and virus isolation, and the serum of patients was tested for HI antibody. The results showed that the H5N1 subtype of patients with tracheal aspirate and the type A influenza virus specific nucleic acid were positive, and the bird flu virus A / Zhejiang / 16/06 (H5N1) strain was isolated. Two copies of serum H5N1 antibody The titers were 1: 320 and 1: 640, respectively, and were etiologically and serologically confirmed as human bird flu cases. The results of sequencing showed that the A / Zhejiang / 16/06 (H5N1) strain was multiple basic amino acids at the HA cleavage site, which was in line with the characteristics of HPAI virus. The HA, NA, PB2, The sequences of NP, M and NS are all avian origins, highly homologous to the isolates of avian influenza virus from Fujian and Anhui in 2005 in China, but significant to the isolates of avian influenza virus isolated from Vietnam, Thailand and Hong Kong in 1997 difference.