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用PAP免疫组织化学方法研究了鸡胚和雏鸡背根节内5-HT神经细胞的形态与胚胎发育,并在离体细胞培养条件下研究了靶周围组织(皮肤)对其胚胎发育的影响。在鸡腰骶部背根节,5-HT免疫反应阳性细胞最先出现于鸡胚13d(E_(13)),占0.4%;孵出后2d(AH_2)占6.2%。阳性细胞主要为大的A类细胞和极少的小B类细胞。免疫反应阳性的周围神经末梢位于皮肤和跟腱中。取自E_9鸡胚的背根节细胞培养7d后有部分出现5-HT阳性免疫反应,取自E_6鸡胚的背根节细胞培养10d仍为阴性反应;然而,当取自E_6鸡胚的背根节细胞与皮肤组织联合培养10d后,则出现5-HT阳性免疫反应细胞。本文还对脊髓5-HT细胞的出现进行了讨论。
PAP immunohistochemistry was used to study the morphology and embryonic development of 5-HT neurons in the dorsal root ganglia of chick embryos and chickens. The effects of the surrounding tissue (skin) on the embryonic development were studied in vitro. In chicken lumbosacral dorsal root ganglion, 5-HT immunoreactive positive cells first appeared on embryo 13d (E_ (13)), accounting for 0.4%; 2 days after hatching (AH_2) accounted for 6.2%. Positive cells are mainly large A-type cells and very few small B-type cells. Immune-positive peripheral nerve endings are located in the skin and Achilles tendon. After 7 days of culture, dorsal root ganglion cells from E_9 chicken embryos showed partial 5-HT positive immune response. Dorsal root ganglion cells from E_6 chicken embryos were still negative for 10 days. However, After cultured with root ganglion and skin tissue for 10 days, 5-HT positive immunoreactive cells appeared. The article also discusses the appearance of spinal cord 5-HT cells.