论文部分内容阅读
用放射性配体分析法测定了胃癌细胞株ＳＧＣ－７９０１、ＮＫＭ－４５的ＬＤＬ受体、ＬＤＬ内移及降解量，并与正常的胃粘膜细胞和建株的人羊膜细胞对照。结果表明，胃癌细胞株ＬＤＬ受体的Ｋｄ值与对照细胞相似，最大结合量较正常细胞显著增加，分别为４１２．０２（ＳＧＣ－７９０１）、３８４．４３（ＮＫＭ－４５）、２９１．０７（胃粘膜细胞）及２９１．４２μｇ／ｇ细胞蛋白（羊膜细胞），胃癌细胞的ＬＤＬ内移及降解量较正常细胞明显增加。提示胃癌细胞株ＳＧＣ－７９０１及ＮＫＭ－４５受体介导的ＬＤＬ代谢率增强，ＬＤＬ受体数量增加，受体对ＬＤＬ的亲和性、特异性与正常细胞之间差异无显著性。
Radioligand analysis was used to determine the inward migration and degradation of LDL receptors and LDL in gastric cancer cell lines SGC-7901 and NKM-45, and was compared with normal gastric mucosa cells and human amniotic cells of established strains. The results showed that the Kd value of LDL receptors in gastric cancer cell lines was similar to that of control cells, and the maximum binding amount was significantly increased compared with normal cells, which were 412.02 (SGC-7901), 384.43 (NKM-45), 291.07 ( Gastric mucosal cells) and 291.42 μg/g cell protein (amniotic cells), LDL migration and degradation of gastric cancer cells increased significantly compared with normal cells. It was suggested that the metabolic rate of LDL mediated by gastric cancer cell lines SGC-7901 and NKM-45 receptors was increased, the number of LDL receptors increased, and there was no significant difference in the affinity, specificity of receptors on LDL and normal cells.