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目的将携带特异性抗体的杂交瘤细胞作为天然抗体＿载体，进行血清学相关抗原检测的方法学研究。方法运用特异性杂交瘤细胞ＥＬＩＳＡ双抗体夹心法，测定一组消化道癌标本包括胃癌６９例，其他消化道癌６３例，胃良性病３５例，以及健康体检者８４例。结果正常值判定为００７６±００７６（ｘ±２ｓ），特异性阻断试验为７３％，回收率为６８４％，批内ＣＶ值为５２％－８９％，批间ＣＶ值为１１７％－１７６％。结论特异性杂交瘤细胞ＥＬＩＳＡ方法测定血清中肿瘤相关抗原，方法简单，具有较强的特异性及敏感性，可应用于临床。
Objective To use the hybridoma cells carrying specific antibodies as natural antibody vectors to carry out methodological studies of serum-associated antigen detection. Methods A specific hybridoma ELISA double antibody sandwich method was used to determine a group of gastrointestinal cancer specimens including 69 cases of gastric cancer, 63 cases of other gastrointestinal cancer, 35 cases of benign stomach disease, and 84 cases of healthy physical examination. Results The normal value was judged to be 0076±0076 (x±2s), the specific blocking test was 73%, the recovery rate was 684%, and the intra-assay CV value was 52%-8.9%. The inter-assay CV value was 11.7%-17-17%. Conclusion The specific hybridoma ELISA method for the determination of tumor-associated antigen in serum is simple, has strong specificity and sensitivity, and can be used clinically.