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目的 采用酶联免疫吸附测定 (ELISA)技术检测疟疾媒介按蚊环子孢子蛋白 (CSP) ,评价ELISA用于云南疟疾媒介按蚊传疟效能调查的可行性。 方法 现场捕获疟疾媒介按蚊经产蚊 ,每只蚊镜检 3叶唾腺子孢子感染率 ,ELISA检测另 3叶唾腺CSP ;用含间日疟原虫配子体的患者血人工喂饲微小按蚊 ,11d后同法镜检唾腺子孢子感染率及ELISA检测唾腺CSP。在种植场捕获 8种按蚊 (微小按蚊、中华按蚊、多斑按蚊、迷糊按蚊、可赫按蚊、带足按蚊、菲律宾按蚊和须喙按蚊 )各龄成蚊 ,ELISA检测唾腺CSP。 结果 ①现场捕获经产蚊 10 10只 ,镜检唾腺子孢子阳性 7只 ,阳性率为 0 69% ;ELISA检测唾腺CSP阳性 8只 ,阳性率为 0 79% ;其中 6只蚊两项检测均为阳性 ,阳性率为 0 5 9%。两法比较 ,差异无显著性意义 (P >0 0 5 )。②人工感染 3 6只微小按蚊 ,镜检唾腺子孢子阳性2 7只 ,阳性率为 75 0 % ;ELISA检测唾腺CSP阳性 2 9只 ,阳性率为 80 6% ;其中有 2 6只蚊两项检测 (Pv2 10CSP)均阳性 ,阳性率为 72 2 %。两法比较 ,差异无显著性意义 (P >0 0 5 )。③种植场捕获 8种按蚊各龄成蚊 4675只 ,ELISA检测唾腺CSP阳性 11只 ,阳性率为 0 2 4% ;其中Pv2 10阳性 9只 ,Pf2A10阳性 2只 ;微小按蚊、中华按蚊和多斑按蚊ELISA检测阳性
Objective To detect the circumsporozoite sporozoite protein (CSP) of Anopheles sinensis using enzyme-linked immunosorbent assay (ELISA) and evaluate the feasibility of ELISA in malaria vector screening. Methods The incidence of salivary gland sporozoites infection in mosquitoes of Malagasy mosquitoes was detected in the field by 3 mosquito malaria test and CSP of the other 3 salivary glands were detected by ELISA. , 11d after the same method of microscopic examination of salivary gland sporozoites infection rate and ELISA detection of salivary gland CSP. Eight adult mosquitoes of the anopheles Anopheles minimus, Anopheles sinensis, Anopheles maculata, Anopheles dirus, Anopheles stephensi, Anopheles stephensi, Anopheles stephensi and Anopheles stephensi were captured at the plantations, ELISA for Salivary CSP. Results ①10 10 mosquito-producing mosquitoes were collected on the spot, and 7 were positive for salivary gland spores microscopy. The positive rate was 0 69%. 8 positive CSPs in salivary glands were detected by ELISA with a positive rate of 0 79% Tests were positive, the positive rate of 0 59%. There was no significant difference between the two methods (P> 0.05). ② Artificially infected 36 Anopheles sinensis, 27 of them were positive for microscopic examination of sporozoites, the positive rate was 75 0%; 29 positive for CSP in salivary glands were detected by ELISA, the positive rate was 80 6%; of which 26 Mosquito two tests (Pv2 10CSP) were positive, the positive rate of 72 2%. There was no significant difference between the two methods (P> 0.05). (3) 4675 adult mosquitoes were collected from 8 species of Anopheles at each planting site, 11 positive CSPs were detected by ELISA, and the positive rate was 0 2 4%. Among them, 9 were Pv2 10 positive and 2 were Pf2A10 positive. Mosquitoes and Anopheles mosquito ELISA test positive