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采用循环伏安法、差分脉冲法研究了木犀草素在多壁碳纳米管修饰碳糊电极上的电化学行为。在p H 3.29的B-R缓冲体系中,木犀草素在0.502 V和0.452 V处产生一对明显的氧化还原峰,其峰电流与木犀草素浓度在5.0×10~(-8)~1.0×10~(-5)mol/L范围内呈良好的线性关系,木犀草素在电极上是受吸附控制的2质子、2电子电极反应过程;在此基础上研究了木犀草素与DNA的相互作用,随着DNA的浓度增加木犀草素的电化学信号逐渐降低,表明木犀草素能与DNA发生明显的相互作用,进一步研究表明木犀草素与DNA的相互作用是以嵌入方式为主。
The electrochemical behavior of luteolin in the multi-walled carbon nanotubes modified carbon paste electrode was studied by cyclic voltammetry and differential pulse method. In the BR buffer system of p H 3.29, luteolin produced a pair of obvious redox peaks at 0.502 V and 0.452 V with a peak current of luteolin at a concentration of 5.0 × 10 -8 to 1.0 × 10 ~ (-5) mol / L, and the luteolin was adsorbed on the electrode by the 2-proton and 2 -electrode reaction processes. On the basis of this, the interaction between luteolin and DNA was studied , The electrochemical signal of luteolin gradually decreased with the increase of DNA concentration, indicating that luteolin could interact with DNA significantly. Further studies showed that the interaction between luteolin and DNA was based on the embedding method.