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2002—2003年采集了黄淮中北部地区为主的32个县市的大豆SMV病样981份,经初步繁殖鉴定、生物纯化及DAS-ELISA血清学检测,得到95个SMV分离物,根据95个分离物在27个鉴别寄主上的症状反应,最终确定南农1138-2、诱变30、8101、铁丰25、Davis、Bu llfalo、早熟18、Kwanggyo、齐黄1号和科丰1号10个品种作为该地区SMV株系的鉴别寄主。利用它们可将95个SMV分离物划分成10个株系群,其中5个与以往鉴定株系群相同,另5个为新发现的株系群,分别将5个新株系群定名为SC-11~SC-15。研究还发现,SC-11株系群所占比例最大,是本地区优势株系;其次为SC-8株系群;SC-11和SC-13株系群分布最广,在黄淮北部各省均有分布。
A total of 981 SMV isolates were collected from 32 counties in central-northern Huanghuai region from 2002 to 2003. 95 SMV isolates were obtained after preliminary breeding identification, biological purification and DAS-ELISA serological tests. Symptoms and reactions of 27 subjects were identified, Nannong 1138-2, Mutagens 30,8101, Tiefeng 25, Davis, Bulfelo, Early Premature 18, Kwanggyo, Qihuang 1 and Kefeng 1 Varieties serve as the identification host for the SMV strain in the area. Using them, 95 SMV isolates could be divided into 10 groups, of which 5 were the same as the previously identified strains and 5 were newly discovered. Five new strains were named as SC- 11 ~ SC-15. The study also found that SC-11 strains accounted for the largest proportion, is the dominant strains in the region; followed by the SC-8 strain group; SC-11 and SC-13 strains most widely distributed in northern Huanghuai provinces All have distribution.