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对中药牡丹皮炭的化学成分进行深入研究,并对其凝血活性进行筛选.通过聚酰胺、硅胶、葡聚糖凝胶等柱色谱方法进行分离纯化,利用核磁共振波谱等方法鉴定结构.共分离鉴定了10个化合物,分别为3-(2-呋喃)-1-(2-羟基-4-甲氧基苯基)-2-丙烯酮(1)、2-(2,5-羟基-4-甲基苯基)丙酸乙酯(2)、5-十四烯酸甲酯(3)、1,4-二乙基-环己烷(4)、儿茶酚(5)、对羟基苯甲酸甲酯(6)、3-羟基-2-甲基-4-吡喃酮(7)、3,8-二羟基-2-甲基色酮(8)、3β-羟基-齐墩果烷-12-烯(9)、1-亚油酸单甘油酯(10).其中1,2为新天然产物,3-10为首次从牡丹皮炭中分离得到.在体外评估化合物潜在的凝血活性,化合物2,9和10具有强烈的止血作用,而化合物1和8可以活化血液循环.“,”The chemical constituents of Cortex Moutan charcoal were studied in depth,and their coagulation activity was screened.The chemical constituents were isolated by polyamide,silica gel and Sephadex G-Sepharose chromatography purification,and their structures were identified by NMR spectroscopy and other analyses.A total of 10 compounds were obtained and identified as follows:3-(2-furan)-l-(2-hydroxy-4-methoxy-phenyl)-2-propylene ketone (1),2-(2,5-hydroxy-4-methyl phenyl) propionate ethyl ester (2),methyl tetradec-5-enoate (3),1,4-diethylcyclohexane (4),catechol (5),methyl-4-hydroxybenzoate (6),3-hydroxy-2-methyl-4-pyrone (7),3,8-dihydroxy-2-metyl-chromone (8),3β-hydroxy-olean-12-en (9),1-monolinolein (10).Among them,compounds 1 and 2 were new natural products,and 3-10 were isolated from the Cortex Moutan charcoal for the first time.The potential coagulation activities of compounds were evaluated,and compounds 2,9 and 10 had strong hemostatic effects.While compounds 1 and 8 could significantly activate blood circulation.