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目的探讨正常乳腺上皮细胞经致癌物联合慢性处理后生物学特性的变化。方法使用低剂量(100pmol/L)的化学致癌物4-甲基亚硝胺-1-(3-吡啶)-1-丁酮(NNK)和苯并芘(B[a]P)连续处理人正常乳腺上皮MCF10A细胞,20代后研究细胞是否获得癌细胞相关生物学特性。结果经致癌物低剂量长期处理后的细胞对生长因子的依赖性降低,获得锚定非依赖性生长特性以及在3D细胞外基质培养基上形成不规则的腺泡。结论经100pmol/L NNK和B[a]P长期处理的人正常乳腺上皮MCF10A细胞可获得癌细胞相关特性。
Objective To investigate the biological characteristics of normal breast epithelial cells treated with carcinogens combined with chronic treatment. Methods Human low-dose (100 pmol / L) chemical carcinogens such as 4-methylnitrosamine-1- (3-pyridyl) -1-butanone (NNK) and benzopyrene (B [a] P) Normal breast epithelial MCF10A cells, after 20 generations of cells to obtain cancer-related biological characteristics. Results After long-term treatment of carcinogens with low doses, the dependence of growth factors on cells was reduced, anchorage-independent growth characteristics were obtained, and irregular acini was formed on 3D extracellular matrix medium. Conclusion The human normal breast epithelial MCF10A cells chronically treated with 100 pmol / L NNK and B [a] P can acquire cancer cell-related features.