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目的:应用电子顺磁技术(ESR)检测慢性阻塞性肺病(COPD)大鼠血清对部分氧自由基的清除能力,探讨COPD发病过程中氧化应激起到的病理作用;方法:将20只SD大鼠随机分成正常组、模型组,每组10只,以烟熏及气管内滴注脂多糖的方式建立大鼠COPD模型,观察记录大鼠一般状况,采用电子ESR检测大鼠血清对氧自由基清除率。结果:大鼠一般状况、肺功能及组织病理学改变提示COPD模型复制成功。与正常组比较,模型组大鼠血清对超氧自由基对羟自由基清除能力低于正常组(P<0.05),对一氧化氮的清除能力与正常组比较没有统计学差异。结论:氧化应激参与COPD形成过程,是其重要病机之一,并且机体对不同的氧自由基清除能力存在差异。
Objective: To detect the scavenging capacity of some oxygen free radicals in sera of chronic obstructive pulmonary disease (COPD) rats by electronic paramagnetic resonance spectroscopy (ESR) and to explore the pathological role of oxidative stress in the pathogenesis of COPD.Methods: 20 SD The rats were randomly divided into normal group and model group, with 10 rats in each group. COPD model was established by smoking and intratracheal instillation of lipopolysaccharide. The general condition of rats was observed and recorded. The electronic oxygenation Basal clearance rate. Results: The general condition, lung function and histopathological changes in rats suggested that COPD model was successfully replicated. Compared with the normal group, the scavenging ability of superoxide radical to hydroxyl radical in the model group rats was lower than that of the normal group (P <0.05), and the scavenging capacity of nitric oxide was not significantly different from that of the normal group. CONCLUSION: Oxidative stress is involved in the process of COPD formation, which is one of the important pathogenesis. And the ability of the body to scavenge different oxygen free radicals is different.