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目的了解北京市男男性行为人群(MSM)中,29例新确证HIV-1感染者病毒株的基因序列特征。方法对HIV-1感染者进行流行病学调查,采集全血,分离血浆提取病毒核酸,应用套式反转录聚合酶链式反应(nested reverse transcription-polymerase chain reaction,nested RT-PCR)分别扩增gag、env和pol基因的部分片段,并对扩增产物进行测序,用Mega 5.0软件将测序结果与各亚型国际参考株比对,根据基因序列离散度确定系统进化树,综合三个区域的分型结果分析基因型及其特征。结果分析样本中扩增阳性的基因序列,结果表明至少存在5种不同的HIV-1亚型和重组模式,包括3种常见CRF01_AE(48.4%),CRF07/08_BC(24.1%),B/B’亚型(10.3%),以及罕见重组型CRF01_AE/CRF07_BC(6.9%)和CRF01_AE/B(10.3%);V3环顶端四肽分析发现,共有五种类型的顶端四肽:GPGQ、GPGR、GPGG、GPGL和GPGP,主要以GPGQ为主(73.3%);辅助受体分析结果发现,CRF01_AE亚型和CRF01_AE/B亚型的样本中出现了预测为X4嗜性的毒株。结论北京地区MSM人群中29例HIV-1感染者病毒株遗传多样性高,来源复杂,且存在罕见CRF01_AE/CRF07_BC和CRF01_AE/B重组型,应当继续加强HIV-1分子流行病学特征的监测。
Objective To investigate the gene sequence of 29 newly confirmed HIV-1 infected individuals in MSM in Beijing. Methods Epidemiological survey of HIV-1 infected persons was conducted. Whole blood was collected and the viral nucleic acids were extracted from the plasma. Nested reverse transcription-polymerase chain reaction (nested RT-PCR) The partial sequences of gag, env and pol genes were amplified and the amplified products were sequenced. The results of the sequencing were compared with those of international subtypes using Mega 5.0 software. The phylogenetic tree was determined according to the genetic sequence divergence. The three regions The typing results were analyzed for genotypes and their characteristics. Results Analysis of the amplified positive gene sequences in the sample showed that there were at least 5 different HIV-1 subtypes and recombination patterns including 3 common CRF01_AE (48.4%), CRF07 / 08_BC (24.1%), B / B ’ (10.3%), and rare recombinant CRF01_AE / CRF07_BC (6.9%) and CRF01_AE / B (10.3%). The analysis of the V3-loop aptamer showed that there are five types of apical tetrapeptides: GPGQ, GPGR, GPGG, GPGL and GPGP, mainly GPGQ (73.3%); co-receptor analysis found that CRF01_AE subtype and CRF01_AE / B subtype samples appeared in the prediction of X4 tropism strains. CONCLUSION: The 29 HIV-1-infected isolates from MSM population in Beijing are characterized by high genetic diversity, complicated origin, and rare CRF01_AE / CRF07_BC and CRF01_AE / B recombinants. HIV-1 molecular epidemiological surveillance should continue to be strengthened.