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目的对金胺“O”荧光染色法在结核病细菌学检验中的临床价值进行分析探讨。方法选择2014年6月-2016年5月在我院检验科接受结核病细菌学检验的疑似结核病患者1230例,所有患者收集3份痰(即时痰、清晨痰、夜间痰),每份标本均经过预处理后制作两张涂片,分别进行金胺“O”荧光染色法与萋-尼染色法检查,最后分别用荧光显微镜和光学显微镜观察抗酸杆菌数量,并对结果进行比较分析。结果 1萋-尼染色法检出抗酸杆菌282例,阳性率为22.93%;金胺“O”荧光染色法检出抗酸杆菌355例,阳性率为28.86%。两种方法比较,差异无统计学意义。2萋-尼染色法检测结果为<1+、1+、2+、3+、4+的标本分别为33例、162例、59例、35例、26例,金胺“O”荧光染色法检测结果为<1+、1+、2+、3+、4+的标本分别为50例、184例、60例、35例、26例。两者比较,萋-尼染色法检测结果为<1+和1+的例数均低于金胺“O”荧光染色法,(P<0.05),差异具有统计学意义。其余检测结果的例数基本相同,差异无统计学意义。结论金胺“O”荧光染色法具有相对较高的检出率,操作简便快捷,有利于批量检验;而且能够显著提高低含菌量标本的检出率,灵敏度较高,可在结核患者的早期诊断治疗中加以推广和应用。
Objective To analyze and discuss the clinical value of gold amine “O” fluorescence staining in bacteriological examination of tuberculosis. Methods From January 2014 to May 2016, 1230 suspected tuberculosis patients receiving tuberculosis bacteriology test in our laboratory were collected. All patients had 3 sputum samples (instant sputum, morning sputum and nocturnal sputum), and each sample passed After the pretreatment, two smears were made. The gold amine “O” fluorescence staining and 萋 -nitrogen staining were used respectively. Finally, the number of acid-fast bacilli was observed by fluorescence microscope and light microscope, respectively, and the results were compared. Results 282 cases of acid-fast bacilli were detected by 萋-nylon staining, the positive rate was 22.93%. 355 cases of acid-fast bacilli were detected by gold amine “O” fluorescence staining, the positive rate was 28.86%. There was no significant difference between the two methods. The results of 2 萋-Ni staining were <1 +, 1 +, 2 +, 3 +, and 4 + were 33, 162, 59, 35, 26 Fluorescent staining test results were <1 +, 1 +, 2 +, 3 +, 4 + specimens were 50 cases, 184 cases, 60 cases, 35 cases, 26 cases. The difference between the two methods was statistically significant (P <0.05). The results of 萋 - Ni stain test were less than that of Auramine and “O” fluorescence staining (P <0.05). The difference was statistically significant. The rest of the test results of the same number of cases, the difference was not statistically significant. Conclusion Auramine “O” fluorescence staining method has a relatively high detection rate, easy and quick to operate, which is good for batch test. It can also significantly improve the detection rate of low-content bacteria with high sensitivity, Patients in the early diagnosis and treatment to be promoted and applied.