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以假孕大鼠为实验对象，在体注射ＩＦＮ－γ研究ＩＦＮ－γ对血浆孕酮水平的影响；黄体器官离体培养研究ＩＦＮ－γ、ＰＧＦ２α对黄体孕酮水平的作用．结果表明：注射ＩＦＮ－γ后，能提高血浆孕酮水平，由８３．８７ｎｇ／ｍＬ升至１１７．０９ｎｇ／ｍＬ，增长３９．６％（Ｐ＜０．０５）；离体培养条件下加入ＩＦＮ－γ（１２０Ｕ／ｍＬ）后也能提高培养液中的孕酮水平（Ｐ＜０．０１），加入ＰＧＦ２α（１００ｎｇ／ｍＬ）则能显著降低培液中的孕酮水平，而同时加入ＩＦＮ－γ（１２０Ｕ／ｍＬ）和ＰＧＦ２α（１００ｎｇ／ｍＬ）时，培养液中孕酮水平明显高于只加ＰＧＦ２α组．可见ＩＦＮ－γ有提高黄体孕酮分泌水平的作用，而且这种作用与抑制ＰＧＦ２α的溶黄体作用有密切关系．
The effects of IFN-γ on progesterone in plasma were studied in vivo. The effects of IFN-γ and PGF2α on the progesterone level were studied in vitro. The results showed that the plasma progesterone level increased from 83.87ng / mL to 117.09ng / mL, increased by 39.6% (P <0.05) after IFN-γ injection. IFN (P <0.01). The addition of PGF2α (100ng / mL) could significantly reduce the level of progesterone in the culture medium, while the addition of IFN- γ (120U / mL) and PGF2α (100ng / mL), the progesterone level in the culture medium was significantly higher than that in the PGF2α-only group. It can be seen that IFN-γ can enhance the secretion of progesterone in the corpus luteum, and this effect is closely related to the inhibition of the role of PGF2α in the dissolution of corpus luteum.