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本实验对经典的细胞转化实验进行了改进，应用甲基丙烯酸环氧丙酯（简称ＧＭＡ）多次作用，建立了人胚肺成纤维细胞转化系统。观察了人胚肺成纤维细胞恶性转化过程中细胞形态和原癌基因ｃ－ｍｙｃ的改变。结果表明：在ＧＭＡ的多次作用下，细胞的恶性程度逐渐增高，形态上由正常到开始排列紊乱，最终形成大量细胞致密堆集的转化灶。经多次染毒后形成的转化细胞在含３％小牛血清的培养基中生长旺盛，其原癌基因ｃ－ｍｙｃ的ｍＲＮＡ表达水平显著增高。本文探讨了人细胞形态及基因的改变与细胞转化乃至肿瘤发生的关系。
In this experiment, the classical cell transformation experiment was improved, and the human embryonic lung fibroblast transformation system was established by using multiple glycidyl methacrylate (abbreviated as GMA). The changes of cell morphology and proto-oncogene c-myc during malignant transformation of human embryonic lung fibroblasts were observed. The results showed that under the multiple actions of GMA, the malignancy of the cells gradually increased, and the morphology was disordered from the normal to the beginning, resulting in the formation of a large number of densely packed transformation foci. The transformed cells formed after multiple exposures grew vigorously in a culture medium containing 3% calf serum, and the expression level of the proto-oncogene c-myc mRNA was significantly increased. This article discusses the relationship between human cell morphology and gene changes and cell transformation and even tumorigenesis.