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表皮生长因子(epidermal growth factor,EGF)是一种含53个氨基酸残基的多肽,分子量6.045ku。已知EGF可促使新生大鼠肝细胞的分裂,并刺激肝细胞DNA的合成。在这些肝细胞的表面已证明具有EGF的受体,Mullhaupt等证明肝有EGF的RNA表达。但至今为止有关EGF在肝癌细胞的定位尚未见报道。Gusterson等发现EGF受体在人肝癌细胞的分布,但对EGF受体在癌细胞的具体定位无详细描述。本研究以培养的大鼠肝癌细胞系为研究对象。采用免疫细胞化学方法观察了EGF及其受体在肝癌细胞的分布,为进一步研究EGF在肝癌细胞中的意义提供形态学依据。
Epidermal growth factor (EGF) is a polypeptide containing 53 amino acid residues with a molecular weight of 6.045ku. It is known that EGF promotes the division of hepatocytes in neonatal rats and stimulates DNA synthesis in hepatocytes. On the surface of these hepatocytes have been shown to have receptors for EGF, and Mullhaupt et al. demonstrated that the liver has EGF RNA expression. However, the location of EGF in liver cancer cells has not been reported so far. Gusterson et al. found that the distribution of EGF receptors in human hepatoma cells, but no detailed description of the specific location of EGF receptors in cancer cells. In this study, the cultured rat hepatoma cell line was studied. The distribution of EGF and its receptor in hepatocellular carcinoma cells was observed by immunocytochemistry, providing morphological basis for further research on the significance of EGF in hepatoma cells.