论文部分内容阅读
胚胎植入和胎盘形成涉及细胞外基质的降解和重建,以及细胞的增殖、凋亡、迁移和分化,基质金属蛋白酶(MMPs) 是参与这些事件的主要蛋白水解酶系统. MMP-26是近年来发现的MMPs家族的新成员,但其功能所知甚少. 通过半定量RT-PCR、免疫组织化学、荧光免疫细胞化学等手段,发现人胎盘中MMP-26主要定位于绒毛滋养层细胞,在绒毛间质细胞中也有少量表达. 妊娠早期,胎盘中MMP-26表达水平较高,至妊娠中期降至最低,但在足月胎盘中其表达又有显著提高,提示MMP-26可能参与妊娠早期滋养层细胞的侵润和分娩时的胎盘剥离. 体外培养的妊娠早期人细胞滋养层细胞能产生一定水平的MMP-26,而其表达受到激活素A的剂量依赖性刺激,表明滋养层细胞中存在MMP-26表达的自分泌/旁分泌调节.
Embryo implantation and placenta formation involve the degradation and remodeling of extracellular matrix, as well as cell proliferation, apoptosis, migration and differentiation, and matrix metalloproteinases (MMPs) are the major proteolytic enzymes involved in these events. Found a new member of the MMPs family, but its function is poorly understood.Through semi-quantitative RT-PCR, immunohistochemistry, fluorescence immunocytochemistry and other means, found that human placenta MMP-26 mainly located in the villus trophoblast cells in the Villus interstitial cells are also a small amount of expression.Pregnant, placental MMP-26 expression level is high, until the second trimester of pregnancy, but in the full-term placenta its expression was significantly increased, suggesting that MMP-26 may be involved in early pregnancy Trophoblast invasion and placental detachment during childbirth. In vitro cultured human gestational trophoblast cells produce a certain level of MMP-26, and its expression is stimulated by activin-A in a dose-dependent manner, indicating that in trophoblast cells There is autocrine / paracrine regulation of MMP-26 expression.