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目的 :建立复方玉驹胶囊的鉴别与含量测定方法。方法 :分别以白蚁巢标准药材和乌药内酯为对照 ,采用薄层色谱法进行鉴别 ;反相高效液相色谱法测定含量 ,色谱柱为Shim packCLC ODS柱 ,检测波长 2 0 8nm ,流动相为乙腈 含0 1%磷酸的水 (5 0∶5 0 ) ,柱温为室温 ,流速 0 5ml·min 1。结果 :薄层色谱鉴别法专属性强 ,HPLC法测定含量精密度高 ,重复性好 (RSD <1 5 % ) ,平均回收率为 10 0 8% ,标准曲线回归方程为Y =- 40 19 3 +5 978C ,r =0 9999。结论 :鉴别与含量测定方法简便、准确 ,可用于复方玉驹胶囊的质量控制
Objective : To establish the identification and content determination method of Compound Yuxi Capsule. METHODS: Thin-layer chromatography was used to identify the termite nest standard medicinal materials and aconite lactone as control. The content was determined by reversed-phase high-performance liquid chromatography. The column was a Shim packCLC ODS column with a detection wavelength of 280 nm and mobile phase. The acetonitrile contains 0 1% phosphoric acid in water (50:500), the column temperature is room temperature, and the flow rate is 0 5 ml·min 1 . RESULTS: The TLC method was highly specific. HPLC assay showed high precision, good reproducibility (RSD <15%), average recovery rate was 10 8%, and the standard curve regression equation was Y =- 40 19 3 +5 978C ,r =0 9999. Conclusion :The method of identification and content determination is simple and accurate. It can be used for the quality control of Fufang Yuqi Capsule.