采后‘琯溪蜜柚’果实柠檬酸合成酶基因的克隆与表达分析

• 作 者: 徐世荣 杨华丽 李小婷 张智伟 潘东明
• 机 构: 福建农林大学园艺产品贮运保鲜研究所,福建农林大学园艺学院,
• 出 处: 中国果树
• 发表日期: 2017年03期
• 关键词: 琯溪蜜柚 合成酶基因 ‘琯溪蜜柚’ 采后 返酸 有机酸含量 生物信息学分析 基因表达量 可滴定酸含量 氨基酸序列

为研究柠檬酸合成酶基因与‘琯溪蜜柚’果实“返酸”的关系,以不同贮藏期‘琯溪蜜柚’果实汁胞为材料,克隆了1个柠檬酸合成酶基因,将其命名为CmCS,其含有1 416 bp开放阅读框,编码具有471个氨基酸的蛋白。生物信息学分析表明,CmCS推导的氨基酸序列与甜橙、川桑等植物的相似度皆大于90%,为稳定的亲水蛋白,亚细胞定位预测其定位于线粒体。结果表明,‘琯溪蜜柚’贮藏期间伴随着有机酸含量逐渐增加,CmCS基因表达量整体先升高后降低,推测其对‘琯溪蜜柚’果实采后有机酸代谢具有一定调控作用。

In order to study the relationship between the citrate synthase gene and the fruit acid “back acid” of ’Guanxi honey pomelo’, one citrate synthase gene was cloned from the fruit juice of ’Guanxi honey pomelo’ at different storage periods, Named CmCS, it contains a 1 416 bp open reading frame encoding a protein with 471 amino acids. Bioinformatics analysis showed that the deduced amino acid sequence of CmCS was more than 90% similar to that of sweet orange and Chuan Sang. It was a stable hydrophilic protein and subcellular localization predicted that it located in mitochondria. The results showed that during the storage period, the content of CmCS gene increased firstly and then decreased with the increase of organic acid content during storage. It was presumed that the expression of CmCS gene had a certain regulation effect on the post-harvest organic acid metabolism of ’Yingxiu pomelo’.