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目的探讨双葛解酲丸对酒精性脂肪肝模型的治疗效果。方法将65只SD大鼠分为空白对照组(11只)和酒精性脂肪肝(alcoholic fatty liver,AFL)组(54只),后者给予白酒灌胃建立AFL模型。8周后,处死其中5只(空白对照组1只,AFL组4只)病理组织学检查以验证其造模成功,然后将AFL组进一步分为模型对照组、双葛解酲丸高剂量组、双葛解酲丸中剂量、双葛解酲丸低剂量组和海王金樽治疗组。治疗4周末处死所以大鼠,检测血清肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白介素-6(interleukin-6,IL-6)、痩素(leptin,LEP)、脂联素(adiponectin,ADP)和游离脂肪酸(free fatty acid,FFA)。同时对肝脏进行病理切片组织学检查。结果双葛解酲丸高剂量组大鼠血清IL-6[(62.49±34.16)pg/ml]、TNF-α[(3.25±0.20)ng/ml]、FFA[(572.16±45.37)μmol/L]和LEP[(1.89±0.21)ng/ml]明显低于模型对照组与海王金樽治疗组(P<0.01),血清ADP[(3.05±0.27)mg/ml]明显高于模型对照组[(2.17±0.37)mg/ml]与海王金樽治疗组[(2.69±0.32)mg/ml,P<0.01)],双葛解酲丸对大鼠肝脏的脂肪变性及炎症有明显改善。结论双葛解酲丸对酒精性脂肪肝有治疗作用。
Objective To investigate the therapeutic effect of Shuangge Jieyao Pill on alcoholic fatty liver disease model. Methods Sixty-five SD rats were divided into blank control group (n = 11) and alcoholic fatty liver (AFL group) (n = 54). The latter group was given alcohol to establish AFL model. After 8 weeks, 5 rats in the control group (1 in the blank control group and 4 in the AFL group) were sacrificed to verify the success of the modeling. Then the AFL group was further divided into model control group, , Shuangge Jiewan pills in medium dose, Shuangge Jiewan pills low-dose group and the sea king Jinzun treatment group. The rats were sacrificed at the end of the 4th week for treatment. Serum levels of TNF-α, IL-6, LEP, Adiponectin (ADP) and free fatty acid (FFA). Liver biopsy at the same time histological examination. Results The serum levels of IL-6 [(62.49 ± 34.16) pg / ml], TNF-α [3.25 ± 0.20 ng / ml] and FFA [(572.16 ± 45.37) μmol / L ] And LEP [(1.89 ± 0.21) ng / ml] were significantly lower than those in the model control group and the sea king jinzun group (P <0.01). Serum ADP was significantly higher than that of the model control group [(3.05 ± 0.27) mg / (2.17 ± 0.37) mg / ml], and Ha Wang Jinzun treatment group (2.69 ± 0.32 mg / ml, P <0.01). The effect of Shuangge Jieyao Pill on steatosis and inflammation in rats’ liver was improved. Conclusion Shuang Ge Jie Jue Wan treatment of alcoholic fatty liver has a therapeutic effect.