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我们对280例不育妇女进行了血清抗子宫内膜抗体(EMAb)、抗精子抗体(ASAb)和抗弓形体抗体(ATAb)的检测及分析。现报告如下。一、资料与方法 1.研究对象:对照组为45名体检健康的孕妇,孕周为24～32。病人组包括:不孕组63例,为婚后2～10年性生活正常未孕妇女,腹腔镜检查未发现异常;流产组47例,为婚后2～14年间,发生自然流产2～次的妇女,妇科检查未发现器质性病变;子宫内膜异位症(EMT)34例经腹腔镜诊断;早产20例、死胎15例、胎儿畸形17例、子宫肌瘤28例、卵巢肿瘤25例和月经不调31例均经临床确诊。收集血清待检。 2.研究方法:EMAb、ASAb、ATAb检测按文献方法进行,为ELISA间接法。分别以子宫内膜抗原(1:400),人精子膜抗原(1:100)和弓形虫抗原(1:100)包被聚苯乙烯反应板,4℃过夜;常法洗孔,均用含15％小牛血清的PBS-T封闭,43℃ lh;洗后分别加入待测血清
280 infertile women were tested for serum anti-endometrial antibody (EMAb), anti-sperm antibody (ASAb) and anti-toxoplasma antibody (ATAb). The report is as follows. First, the data and methods 1 research object: control group of 45 healthy pregnant women, gestational age is 24 to 32. The patient group included: Infertility group 63 cases, 2 to 10 years after marriage, normal sexual women who were not pregnant, laparoscopy found no abnormalities; 47 cases of abortion, 2 to 14 years after marriage, spontaneous abortion occurred 2 ~ times Of women, gynecological examination found no organic lesions; endometriosis (EMT) 34 cases diagnosed by laparoscopy; premature birth in 20 cases, 15 stillbirths, fetal malformations in 17 cases, 28 cases of uterine fibroids, ovarian tumors 25 Cases and irregular menstruation 31 cases were clinically diagnosed. Collect the serum to be tested. 2. Research Methods: EMAb, ASAb, ATAb detection by literature methods, indirect ELISA. Polystyrene reaction plates were coated with endometrial antigen (1: 400), human sperm membrane antigen (1: 100) and Toxoplasma gondii antigen (1: 100) overnight at 4 ° C. 15% fetal bovine serum in PBS-T blocked, 43 ℃ lh; washed were added to the serum to be tested