论文部分内容阅读
目的:本研究旨在分析孕鼠暴露于全氟辛烷磺酸(potassium perfluorooctanesulfonate,PFOS)后对仔鼠脑组织蛋白成分的影响.方法:将孕3 d母鼠随机分为2组.正常组孕鼠给予溶剂生理盐水灌胃;染毒组孕鼠给予0.5 mg/kg PFOS灌胃染毒,染毒连续进行7 d.收集正常组所产仔鼠(A组)与0.5 mg/kg PFOS染毒组所产仔鼠(B组)出生后24 h内全脑组织,使用蛋白组学shotgun法进行脑组织蛋白成分鉴定并分析.结果:鉴定结果显示染毒组所产仔鼠脑组织蛋白种类相比于正常组数量减少,且有无功能蛋白产生.结论:孕鼠PFOS染毒对其仔鼠脑内蛋白质的种类组成有明显影响.“,”Objective:To clarify the changes in the filial mice brain proteins after the pregnant mice exposure to Potassium perfluorooctanesulfonate(PFOS).Methods:The 3 days pregnant mice were randomly divided into 2 groups.The mice in the control group were orally administered with the normal saline.The mice in the other group were orally administered with 0.5mg/kg PFOS for 7 days.The filial mice(within 24 hours) born from the control group(marked as A group) and 0.5 mg/kg PFOS-exposed group(marked as B group) were killed and the brain proteins were collected and homogenized respectively.Changes in brain proteins of the filial mice of A group and B group were examined by shotgun analysis.Results:Compared with A group,the categories of brain proteins in B group were significantly changed and some uncharacterized proteins were detected.Conclusion:Orally administration of PFOS to the pregnant mice results in the abnormal changes in the brain proteins of the filial mice.