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目的 研究胸苷酸合成酶 (TS) 3’ UTR基因多态和生活习惯及亚甲基还原酶基因相互作用与胃癌易感性的关系。方法 在上消化道癌高发区淮安市进行病例对照研究 (胃癌患者 1 0 7例 ,人群对照 2 0 0名 ) ,调查研究对象的生活习惯 ,采用聚合酶链反应 限制性片段长度多态性 (PCR RFLP)技术检测研究对象的TS 3’ UTR基因型。结果 (1 )胃癌组中TS +6bp +6bp、+6bp - 6bp、- 6bp - 6bp基因型频度分别为5 .6 %、47.7%和 46 .7% ,与对照组的 9.0 %、54 .0 %和 37.0 %相比 ,差异无显著性 (χ2 =3 .1 90 ,P =0 .2 0 3)。与携带 +6bp等位基因者相比 ,携带TS - 6bp - 6bp基因型者胃癌发生的危险性有所升高 (调整OR =1 .36 ,95 %CI:1 .0 0～ 1 .78,P =0 .0 4 7)。 (2 )TS - 6bp - 6bp基因型对吸烟、饮酒及不饮茶的习惯在胃癌发生中的作用有放大效应。与携带 +6bp等位基因且不吸烟者或不经常饮酒者或饮茶者相比 ,携带 - 6bp - 6bp基因型且有吸烟习惯者或经常饮酒者或不饮茶者发生胃癌的调整OR分别为 2 .79(95 %CI :1 .51～ 5 .1 8)、1 .76(95 %CI:1 .0 7～ 2 .90 )和 2 .34(95 %CI :1 .43～ 3 .82 )。 (3)TS - 6bp - 6bp基因型与亚甲基四氢叶酸还原酶 (MTHFR)C T或T T基因型有协同作用。携带TS - 6bp - 6bp基因型同时
Objective To study the relationship between polymorphism of 3 ’UTR gene of thymidylate synthase (TS) and lifestyle and methylenetransferase gene interaction and gastric cancer susceptibility. Methods A case-control study was conducted in Huai’an City, a high incidence area of upper gastrointestinal cancer (107 cases of gastric cancer and 200 controls in population). The living habits of the subjects were investigated. The restriction fragment length polymorphism (PCR) PCR RFLP) technique to test the TS 3 ’UTR genotype of the study subjects. Results (1) The frequencies of TS + 6bp + 6bp, + 6bp - 6bp and - 6bp - 6bp in gastric cancer group were 5.6%, 47.7% and 46.7%, respectively, compared with 9.0% and 54% in control group. There was no significant difference between 0% and 37.0% (χ2 = 3.190, P = 0.203). Compared with those carrying the + 6bp allele, the risk of gastric cancer with TS - 6bp - 6bp genotype increased (adjusted OR = 1.36, 95% CI: 1.0-0.178, P = 0 .0 4 7). (2) The effect of TS - 6bp - 6bp genotype on smoking, drinking and not drinking tea has a magnifying effect on the occurrence of gastric cancer. Adjusted OR of gastric cancer with - 6bp - 6bp genotype with a smoking habit or with or without frequent drinking, compared with those carrying the + 6bp allele and not smokers or infrequent drinkers or tea drinkers, respectively Were 2.79 (95% CI: 1.51-5.18), 1.76 (95% CI: 0.07-2.90) and 2.34 (95% CI: 1.43-3 .82). (3) The TS - 6bp - 6bp genotype has a synergistic effect with CTH or T T genotype of methylenetetrahydrofolate reductase (MTHFR). Carrying TS - 6bp - 6bp genotype simultaneously