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目的分析多烯紫杉醇联合微波热疗对鼻咽癌小鼠免疫功能的影响,为鼻咽癌的临床治疗提供指导帮助。方法将60只SPF级的雌性Balb/c小鼠,采用随机数字表法分为健康对照组、多烯紫杉醇对照组、多烯紫杉醇组、热疗对照组、热疗组及多烯紫杉醇联合热疗组,每组10只。除健康对照组外,其余50只小鼠进行鼻咽癌建模,建模完成后多烯紫杉醇对照组腹腔注射等量生理盐水,多烯紫杉醇组按20 mg/kg剂量腹腔注射,热疗对照组微波37℃持续30 min治疗(2次),热疗组微波43℃持续30 min治疗(2次),多烯紫杉醇联合热疗组在给药1 h后43℃微波持续30 min治疗(2次)。健康对照组不予处理。观察小鼠的状况;测量其体质量;治疗10 d后用流式细胞仪检测各组小鼠脾脏T淋巴细胞亚群中的CD3~+、CD4~+、CD8~+及CD4/CD8比值,并进行对比。结果健康对照组小鼠活动、饮食、排泄、毛色正常,体形无明显变化;鼻咽癌小鼠建模成瘤率约92.00%(46/50),鼻咽癌小鼠与健康对照组小鼠比较,行动迟缓,饮食下降,皮毛蓬松无光泽。观察小鼠平均体质量发现,多烯紫杉醇联合热疗组下降明显,与其他鼻咽癌组比较差异均有统计学意义(P均<0.05),提示瘤体减小。鼻咽癌小鼠的CD3~+、CD4~+以及CD4/CD8比值与健康对照组小鼠比较均明显下降(P均<0.05),而CD8~+明显上升(P<0.05);多烯紫杉醇联合热疗组小鼠的T淋巴细胞亚群与多烯紫杉醇组比较,差异无统计学意义(P均>0.05),表明小鼠的免疫抑制作用仍然存在;而与热疗组小鼠对比,CD3~+、CD4~+及CD4/CD8比值均下降(P均<0.05),CD8~+上升(P<0.05),表明热疗不会加剧免疫抑制的作用。结论多烯紫杉醇联合热疗并不会改善鼻咽癌小鼠免疫功能,临床治疗仍需使用免疫调节剂,以提高机体免疫功能,从而提高抗肿瘤能力,延长存活时间。
Objective To analyze the effect of docetaxel combined with microwave hyperthermia on the immune function of nasopharyngeal carcinoma mice and provide guidance for the clinical treatment of nasopharyngeal carcinoma. Methods Sixty SPF female Balb / c mice were randomly divided into healthy control group, docetaxel control group, docetaxel group, hyperthermia control group, hyperthermia group and docetaxel combined with heat Treatment group, each group of 10. In addition to the healthy control group, the remaining 50 mice model of NPC, docetaxel control group was injected intraperitoneally with equal volume of normal saline, docetaxel group by 20 mg / kg dose of intraperitoneal injection, hyperthermia control The mice in the hyperthermia group were treated with microwave at 43 ° C for 30 min and the mice in the hyperthermic group were treated with microwave at 43 ° C for 30 min Times). The healthy control group did not deal with it. After 10 days of treatment, the ratio of CD3 +, CD4 +, CD8 + and CD4 / CD8 in splenic T lymphocyte subsets of each group was detected by flow cytometry, And contrast. Results The mice in normal control group had activity, diet, excretion, normal coat color and no significant change in body shape. The nasopharyngeal carcinoma mice were established with tumor formation rate of 92.00% (46/50), and the nasopharyngeal carcinoma mice and healthy control mice Compare, slow, dietary decline, fluffy fur. Observed the average body weight of mice found that docetaxel combined hyperthermia group decreased significantly compared with other nasopharyngeal carcinoma groups were statistically significant (P all <0.05), suggesting that the tumor decreased. The ratio of CD3 ~ +, CD4 ~ + and CD4 / CD8 in nasopharyngeal carcinoma mice was significantly lower than that in healthy control mice (all P <0.05), while CD8 + was significantly increased (P <0.05) There was no significant difference in T lymphocyte subpopulation between mice in combined hyperthermia group and docetaxel group (all P> 0.05), indicating that the immunosuppressive effect still existed in mice; Compared with the mice in hyperthermia group, CD3 ~ +, CD4 ~ + and CD4 / CD8 decreased (all P <0.05) and CD8 + increased (P <0.05), indicating that hyperthermia does not exacerbate immunosuppression. Conclusions Docetaxel combined with hyperthermia does not improve the immune function of mice with nasopharyngeal carcinoma. It is still necessary to use immunomodulatory agents in clinical treatment to improve immune function, thereby enhancing anti-tumor ability and prolonging survival time.