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目的探讨门诊就诊者HIV1/2抗体筛查检测的效果及其分布特点。方法用第四代酶联免疫吸附试验(ELISA)试剂检测HIV1/2的抗原及抗体,阳性的样本经复检后仍为阳性即送杭州市疾病预防控制中心进行免疫印迹(Western blot)确证试验。结果 147 229份血液标本初筛阳性165例,检出率0.11%,确证阳性90例,阳性率0.07%,两者符合率为54.55%。确证阳性样本,筛查检测S/CO平均值为19.34;不确定人数6例,占3.64%,在追踪观察中2例患者2个月后确证为阳性。确证阳性样本逐年上升,且以19~45岁男性居多。结论第四代酶联免疫吸附试验(ELISA)试剂检测HIV1/2抗原及抗体的检测具有很高的敏感性;本地区就诊者中HIV1/2抗体阳性者主要集中在19~45岁男性,应引起相关部门的重视。
Objective To investigate the effect and distribution of HIV1 / 2 antibody screening in outpatients. Methods HIV1 / 2 antigens and antibodies were detected by the fourth generation enzyme-linked immunosorbent assay (ELISA) reagent. The positive samples were still positive after retesting. Hangzhou Center for Disease Control and Prevention was confirmed by Western blot . Results A total of 147 229 blood samples were positive for primary screening in 165 cases, the detection rate was 0.11%. The positive results were 90 and the positive rate was 0.07%. The coincidence rate was 54.55%. Confirmed positive samples, screening S / CO average of 19.34; 6 were uncertain population, accounting for 3.64%, 2 patients in the follow-up observation confirmed positive after 2 months. Confirmed positive samples increased year by year, and 19 to 45-year-old male majority. Conclusions The detection of HIV1 / 2 antigen and antibody by the fourth generation of enzyme-linked immunosorbent assay (ELISA) reagent is highly sensitive. Among the HIV-1/2 antibody positive patients in this region, the majority of men aged 19-45 years should be Aroused the attention of relevant departments.