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目的:探讨microRNA-21表达水平对新疆地区食管癌早期诊断及预后评估的临床价值。方法:收集我院收治的100例食管癌患者,其中哈萨克族患者50例,汉族患者50例,采用RT-PCR以及RT-qPCR的方法对患者血清microRNA-21表达水平进行检测并比较。结果:食管癌患者癌组织中microRNA-21表达水平均高于癌旁组织,差异具有统计学意义(P<0.05);有淋巴结转移、ⅡB+Ⅲ期、低分化癌组织中的miRNA-21相对表达量高于无淋巴结转移、I+ⅡA期以及高分化癌组织患者,差异具有统计学意义(P<0.05);miRNA-21在不同民族、性别、年龄分组中表达无明显差异(P>0.05)。结论:microRNA-21表达水平对新疆地区食管癌患者癌组织中表达水平较高,在有淋巴转移、ⅡB+Ⅲ期以及低分化癌组织中表达水平较高,民族、性别以及年龄对microRNA-21水平的影响较小,因此microRNA-21检测对食管癌的早期诊断及预后判断具有指导意义。
Objective: To investigate the clinical value of microRNA-21 expression in early diagnosis and prognosis of esophageal cancer in Xinjiang. Methods: 100 patients with esophageal cancer were enrolled in our hospital. Among them, 50 were Kazakh patients and 50 were Han patients. The expression of microRNA-21 in serum was detected by RT-PCR and RT-qPCR. Results: The expression levels of microRNA-21 in esophageal cancer tissues were significantly higher than those in paracancerous tissues (P <0.05). There was significant difference in the expression of miRNA-21 in lymph node metastasis, stage ⅡB + Ⅲ and poorly differentiated carcinoma The expression level of miRNA-21 was significantly higher in patients with lymph node metastasis, I + II A, and well-differentiated carcinoma (P <0.05). There was no significant difference in miRNA-21 expression among different ethnic groups, gender and age groups ). Conclusion: The expression level of microRNA-21 is significantly higher in esophageal cancer tissues than that in the esophageal cancer tissues in Xinjiang. The expression level of microRNA-21 is higher in lymph node metastasis, stage ⅡB + Ⅲ and poorly differentiated carcinoma tissues. The microRNA- The impact of microRNA-21 on the early diagnosis and prognosis of esophageal cancer is instructive.