论文部分内容阅读
目的探讨丙烯酰胺(acrylamide,ACR)染毒不同时间对神经组织和血清中NRG1的影响。方法雄性SD大鼠80只,随机分为对照组和丙烯酰胺(ACR)组。丙烯酰胺大鼠腹腔注射20 mg/kg的ACR,每周5次;对照组腹腔注射等量的生理盐水。在ACR染毒后第7、14、21和28天观察大鼠的一般状况,检测大鼠神经行为的变化。免疫组化方法观察大鼠小脑、脊髓、坐骨神经NRG1蛋白定位,并采用ELISA法检测神经组织和血清蛋白NRG1的影响。结果与对照组比较,ACR染毒28 d后大鼠体重出现明显下降;第21和28天时大鼠舔后爪时间明显延长;ACR染毒7 d可见脊髓NRG1显著下降,并持续到染毒28 d;小脑中的NRG1在第21天下降了44.74%,差异有显著性。坐骨神经中NRG1出现先上升,然后呈下降趋势;染毒14 d时血清中NRG1开始下降,其下降趋势与脊髓的变化是一致的。免疫组化结果显示NRG1表达在小脑的分子层、浦肯野细胞和颗粒层细胞、脊髓灰质神经元和白质中神经胶质细胞、坐骨神经的雪旺氏细胞。结论丙烯酰胺导致的神经组织中NRG1的表达显著下降。
Objective To investigate the effects of acrylamide (ACR) on NRG1 in nerve tissue and serum at different time points. Methods Eighty male SD rats were randomly divided into control group and ACR group. Acrylamide rats were intraperitoneal injection of ACR 20 mg / kg, 5 times a week; the control group was injected intraperitoneally with the same amount of saline. On the 7th, 14th, 21st and 28th day after ACR exposure, the general condition of the rats was observed and the changes of neurobehavioral changes were observed. Immunohistochemistry was used to observe the localization of NRG1 protein in the cerebellum, spinal cord and sciatic nerve in rats. The effects of NRG1 on neural tissue and serum protein were detected by ELISA. Results Compared with the control group, the weight of rats decreased significantly after ACR exposure for 28 days. The time of licking the hind paws of rats was obviously prolonged on the 21th and 28th days. The NRG1 level in the spinal cord decreased significantly on the 7th day after ACR exposure and continued to the exposure of 28 d; NRG1 in cerebellum decreased by 44.74% on day 21, the difference was significant. NRG1 in sciatic nerve firstly increased and then decreased, while NRG1 in serum decreased at 14 d after exposure. The decline trend was consistent with that of spinal cord. Immunohistochemical results showed that NRG1 was expressed in the cerebellar molecular layer, Purkinje cells and granulosa cells, spinal gray matter neurons and white matter glial cells, sciatic nerve Schwann cells. Conclusion The expression of NRG1 in nerve tissue induced by acrylamide decreased significantly.