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目的对1株南海沉积环境来源真菌的次生代谢产物进行分离、鉴定及活性研究。方法采用溶剂萃取、硅胶柱层析、凝胶柱层析等方法对真菌Eurotiumsp.SCSIO F452的发酵产物进行化学分离,通过NMR、MS等波谱学技术并参阅文献进行化合物结构鉴定,采用SRB法评价化合物的细胞毒活性。结果从菌株SCSIO F452中分离鉴定6个单体化合物,分别为:isodihydroauroglaucin(1)、flavoglaucin(2)、tetrahydroauro-glaucin(3)、2-(1,1-dimethyl-2-propen-1-yl)-1H-indole-3-carboxaldehyde(4)、neoechinulin A(5)和methyl lino-leate(6)。化合物1-5对4种肿瘤细胞系表现出不同强度的细胞毒活性。结论苯甲醛衍生物1-3是真菌SC-SIO F452的优势代谢产物,细胞毒活性较强,具有潜在的研究价值。
OBJECTIVE To isolate, identify and study the secondary metabolites of fungi from sedimentary environment in the South China Sea. Methods The fermentation products of the fungus Eurotium sp. SCSIO F452 were separated by solvent extraction, silica gel column chromatography and gel column chromatography. The structures of the compounds were identified by NMR, MS and other spectroscopic techniques. The structure of the compounds was identified by SRB method. Cytotoxic activity of compounds. Results Six monomer compounds were isolated and identified from the strain SCSIO F452, namely isodihydroauroglaucin (1), flavoglaucin (2), tetrahydroauro-glaucin (3) and 2- (1,1-dimethyl-2-propen-1-yl ) -1H-indole-3-carboxaldehyde (4), neoechinulin A (5) and methyl lino-leate (6). Compounds 1-5 showed different intensities of cytotoxic activity to the four tumor cell lines. Conclusion Benzaldehyde derivative 1-3 is the dominant metabolite of fungi SC-SIO F452, and has strong cytotoxic activity and potential value.