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目的研究仿刺参糖胺聚糖(HGAG)对小鼠腹腔巨噬细胞(Mφ)功能的影响,探讨其对小鼠的免疫调节作用。方法不同浓度HGAG作用于体外培养Mφ,MTT比色法测定代谢活力;中性红法测定吞噬功能;试剂盒测定Mφ中乳酸脱氢酶(LDH)活性;Griess法测定NO生成量;ELISA法测定培养上清中TNF-α、IL-1β的分泌水平。结果 HGAG在0.1~25μg·mL-1范围内对Mφ的功能有一定的促进作用。在1~10μg·mL-1范围内与空白对照组相比可显著增强Mφ代谢活力和吞噬能力(P<0.01),提高LDH活力和NO生成量(P<0.05),显著增加培养上清中TNF-α和IL-1β(P<0.01)的浓度。与阳性对照组相比,在1~10μg·mL-1范围内对Mφ吞噬功能以及TNF-α、IL-1β的生成有更好的促进作用(P<0.05),在高浓度(100μg·mL-1)Mφ的各项功能指标均低于空白对照组。结论 HGAG在1~10μg·mL-1浓度范围内可显著促进Mφ活性,并在一定程度上优于阳性对照组。表明HGAG能够激活Mφ,促进Mφ的功能,增强机体免疫。
Objective To investigate the effect of glycosaminoglycan (HGAG) on the function of mouse peritoneal macrophages (Mφ) and its immunoregulatory effects on mice. Methods Different concentrations of HGAG were used to culture Mφ in vitro. Metabolic activity was determined by MTT colorimetric assay. Phagocytosis was assayed by neutral red assay. Lactate dehydrogenase (LDH) activity in Mφ was assayed by kit. NO production was measured by Griess assay. The supernatant TNF-α, IL-1β secretion levels. Results HGAG could promote the function of Mφ in the range of 0.1 ~ 25μg · mL-1. Compared with the blank control group, the activity and phagocytosis ability of Mφ in the range of 1 ~ 10μg · mL-1 were significantly increased (P <0.01), LDH activity and NO production were increased (P <0.05) TNF-α and IL-1β (P <0.01). Compared with the positive control group, the phagocytic function of Mφ and the production of TNF-α and IL-1β were promoted in the range of 1 ~ 10μg · mL-1 (P <0.05) -1) Mφ function indicators are lower than the blank control group. Conclusion HGAG can significantly promote Mφ activity in the concentration range of 1 ~ 10μg · mL-1, and to a certain extent, it is superior to the positive control group. It shows that HGAG can activate Mφ, promote Mφ function and enhance immunity.