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放疗时,大部分病人感有急性胃肠道紊乱,诸如腹泻、恶心和里急后重;另有2～5%病人有肠道后遗症,如狭窄、瘘、慢性腹泻和吸收不良等。此类损害的形态学基础有纤维化、闭塞性动脉内膜炎和淋巴水肿。据作者所知,对放射性肠炎中的肠道内分泌细胞迄未进行研究。为此,作者研究了13例放射性肠炎的大、小肠切除标本,取样时保持粘膜完整。另取9倒无组织病理学异常的肠道标本作为对照。所有标本均固定在10%福马林缓冲液中,按常规包埋。内分泌细胞用Grimelius银浸渍和嗜铬粒蛋白(chromsgranin)单克隆抗体免疫组织化学法染色,按1986年已报道的方法定量测定组
Radiotherapy, the majority of patients with acute gastrointestinal disorders, such as diarrhea, nausea and tenesmus; another 2 to 5% of patients with intestinal sequelae, such as stenosis, fistula, chronic diarrhea and malabsorption. Morphological basis for such lesions are fibrosis, obstructive endocarditis and lymphedema. To the author’s knowledge, no studies have been conducted on enteroendocrine cells in radiation enteritis. To this end, the authors studied 13 cases of radioactive enteritis large and small intestine resection specimens, mucosal integrity to maintain sampling. Another nine inverted histopathological abnormal intestinal samples as a control. All specimens were fixed in 10% formalin buffer and routinely embedded. Endocrine cells were stained with Grimelius silver impregnated and chromsgranin monoclonal antibody by immunohistochemistry and quantified according to the method reported in 1986