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目的 研究骨髓基质细胞在向成骨细胞分化的介质中 ,17β 雌二醇 (E2 )对其骨形态发生蛋白受体 (BMPR)ⅠA及核结合因子α1(Cbfα1)mRNA表达的影响 ,探讨雌二醇对成骨细胞生成的作用。方法 用 1,2 5 (OH) 2 D3和地塞米松(DEX)诱导大鼠骨髓基质细胞向成骨细胞分化 ,应用RT PCR和Northernblot技术 ,观察不同浓度E2对骨髓基质细胞分化过程中BMPR ⅠA及Cbfα1mRNA表达的影响 ,以α 磷酸奈酚为底物测定细胞碱性磷酸酶的活性 ,VanGieson染色法显示Ⅰ型胶原的含量。结果 E2能明显抑制骨髓基质细胞向成骨细胞分化过程中BMPR ⅠA及Cbfα1mRNA的表达 ,且呈剂量依赖性 ,并被Northernblot结果所证实。ALP活性随E2浓度增加而降低 ,细胞Ⅰ型胶原的合成量随E2浓度增加而减少。结论 E2能明显抑制体外培养的骨髓基质细胞分化过程中BMPR ⅠA及Cbfα1mRNA的表达 ,降低成骨细胞生成。
Objective To investigate the effect of 17β estradiol (E2) on the expression of ⅠA and Cbfα1 mRNA of bone morphogenetic protein receptor (BMPR) and bone morphogenetic protein-1 (Cbfα1) in bone marrow stromal cells differentiated into osteoblasts. Effect of alcohol on osteoblastogenesis. Methods Rat bone marrow stromal cells were induced to differentiate into osteoblasts with 1,25 (OH) 2 D3 and dexamethasone (DEX). RT-PCR and Northern blotting were used to observe the effect of different concentrations of E2 on the differentiation of bone marrow stromal cells And Cbfα1 mRNA expression. The activity of alkaline phosphatase (ALP) was determined by α-phospholipid as substrate. The content of collagen type Ⅰ was detected by VanGieson staining. Results E2 significantly inhibited BMPR Ⅰ A and Cbfα1 mRNA expression in bone marrow stromal cells in a dose-dependent manner and was confirmed by Northern blot. The activity of ALP decreased with the increase of E2 concentration. The amount of type I collagen decreased with the increase of E2 concentration. Conclusion E2 can significantly inhibit the expression of BMPR Ⅰ A and Cbfα1 mRNA in bone marrow stromal cells and decrease the osteoblast formation in vitro.