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目的观察乙型肝炎核心抗原(hepatitis B core antigen,HBcAg)和乙型肝炎e抗原(hepatits B e antigen,HBeAg)对人树突状细胞的影响。方法分离20例健康者外周抗凝血淋巴细胞,将贴壁细胞加重组人白细胞介素4和重组人粒-巨噬细胞集落刺激因子培养至第7天,将树突状细胞分为HBcAg组、HBeAg组和树突状细胞对照组,分别加入HBcAg、HBeAg对树突状细胞刺激培养至第10天,检测刺激培养后细胞表型与分泌的细胞因子及其对树突状细胞体外诱导的淋巴细胞增殖效应和淋巴细胞毒活性的影响。结果 HBcAg组、HBeAg组树突状细胞表面分子(CD83,CD86,HLA-ABC)水平和分泌干扰素-γ、白细胞介素-12p70水平、体外诱导淋巴细胞增殖效应及淋巴细胞毒活性与对照组比较,差异有统计学意义(P<0.01);HBcAg组增强树突状细胞的分子表达、分泌白细胞介素-12p70及体外诱导淋巴细胞毒活性与HBeAg组比较差异有统计学意义(P<0.01)。结论 HBcAg和HBeAg可增加人树突状细胞表达成熟活化的分子、分泌细胞因子,增强树突状细胞体外诱导淋巴细胞增殖效应及淋巴细胞毒活性。
Objective To observe the effects of hepatitis B core antigen (HBcAg) and hepatitis B e antigen (HBeAg) on human dendritic cells. Methods Peripheral anticoagulant lymphocytes were isolated from 20 healthy donors. Adherent cells were treated with recombinant human interleukin-4 (IL-4) and recombinant human granulocyte-macrophage colony stimulating factor (RAPD) for 7 days. The dendritic cells were divided into HBcAg group , HBeAg group and dendritic cell control group were cultured with dendritic cells (HBcAg and HBeAg) for 10 days respectively. The phenotype and secretion of cytokines and the induction of dendritic cells in vitro Lymphocyte proliferation and lymphocyte cytotoxicity. Results The level of CD83, CD86 and HLA-ABC on the surface of dendritic cells in HBcAg and HBeAg groups and the levels of IFN-γ, IL-12p70, lymphocyte proliferation induced in vitro and lymphocyte cytotoxicity were significantly higher than those in control group (P <0.01). The HBcAg group enhanced the expression of dendritic cells, the secretion of interleukin-12p70 and in vitro induced lymphocyte cytotoxicity were significantly different from those of HBeAg group (P <0.01) ). Conclusions HBcAg and HBeAg can increase the expression of mature activated molecules, secrete cytokines and enhance the lymphocyte proliferation and lymphocyte cytotoxicity induced by dendritic cells in vitro.