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本室所建立的小鼠胸腺上皮细胞株ＭＴＥＣＢ以５．０×１０５细胞／ｍｌ的浓度在含１０％小牛血清的ＤＭＥ培养液中，５％ＣＯ２３７℃条件下，培养６ｄ，离心收集上清液。以ＩＬ－６依赖细胞株采用ＭＴＴ法对ＭＴＥＣＢ的上清液进行ＩＬ－６活性检测。结果发现ＭＴＥＣＢ能自发产生低水平的ＩＬ－６，其活性单位为１９．２Ｕ／ｍｌ。ＭＴＥＣＢ的ＩＬ－６产生量低于文献报道的其他胸腺上皮细胞株，说明我们建立的胸腺上皮细胞株有新的特点，反映出胸腺上皮细胞存在高度异质性。
The established mouse thymus epithelial cell line MTECB was cultured at a concentration of 5.0 × 10 5 cells / ml in DME medium containing 10% fetal bovine serum at 5% CO237 ° C. for 6 days. The supernatant was collected by centrifugation liquid. The supernatant of MTECB was assayed for IL-6 activity by MTT assay in an IL-6-dependent cell line. The results showed that MTECB can spontaneously produce low levels of IL-6, the activity unit is 19.2U / ml. MTECB IL-6 production was lower than other reported thymus epithelial cell lines, indicating that we established a new feature of the thymic epithelial cells, reflecting the high degree of heterogeneity of thymus epithelial cells.