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以细胞转化为实验模型，应用细胞生物学和分子生物学的实验手段，研究了辐射所致人胚肺细胞体外转化以及辐射对细胞ＤＮＡ单链断裂损伤效应的规律。接受０．２５～５Ｇｙ６０Ｃｏγ射线作用后第２０代，各剂量组的人胚肺细胞半固体琼脂培养集落形成能力均明显增加，说明人胚肺细胞在射线作用下发生了转化，而且细胞形态转化的出现具有时相性及作用剂量依赖性。采用缺口翻译技术检测细胞ＤＮＡ单链断裂损伤的结果表明，经不同剂量作用后，反映细胞ＤＮＡ单链断裂损伤程度的３ＨｄＴＴＰ掺入量，在各剂量作用组均明显增加，表明射线造成了细胞ＤＮＡ损伤。ＤＮＡ单链断裂损伤效应与剂量之间的关系亦表现为正相关。统计学分析表明，辐射所致细胞ＤＮＡ单链断裂损伤与辐射诱发的细胞转化之间存在着相关关系，辐射诱发的细胞转化随着细胞ＤＮＡ单链断裂损伤效应的加大而增强。这一结果提示，辐射致癌效应与ＤＮＡ损伤效应密切相关。
The cell transformation into experimental model, the use of experimental methods of cell biology and molecular biology to study the radiation-induced transformation of human embryonic lung cells and radiation on the single-strand DNA damage the rules. At 20 generations after exposure to 0.25-5Gy60Coγ-rays, the colony-forming ability of human embryo lung cells in each dose group increased significantly, indicating that human embryonic lung cells transformed under the action of radiation and the morphological changes of cells Appear to have the phase of the role and dose-dependent. The use of nick translation technology to detect single-strand DNA damage results show that after different doses of DNA single strand breaks reflect the degree of damage 3H-dTTP incorporation at each dose group were significantly increased, indicating that the radiation caused Cell DNA damage. The relationship between DNA single strand breakage damage effect and dose also showed a positive correlation. Statistical analysis showed that there was a correlation between radiation-induced DNA single-strand breaks and radiation-induced cell transformation. Radiation-induced cell transformation increased with DNA single-strand breaks. This result suggests that the carcinogenic effect of radiation is closely related to the DNA damage effect.