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目的:观察三化汤对脑缺血再灌注大鼠神经功能、脑含水量及脑组织病理形态学的影响。方法:将大鼠随机分为假手术组、模型组、三化汤低、高剂量组(7.2,14.4 g.kg-1)ig、尼莫地平组(8.1 mg.kg-)1ig,每日1次,连续7 d,给药1周后采用线栓法制备脑缺血再灌注大鼠模型。缺血2 h再灌注24 h后按照Zealonga 5级法对大鼠神经受损症状评分;并取脑称量脑湿重和脑干重,计算脑含水量;HE染色分析脑组织病理学改变。结果:与假手术组比,模型组大鼠神经受损症状严重(P<0.01),脑含水量明显升高(P<0.01),光镜见神经细胞明显肿胀、变性、坏死、炎细胞浸润及血管扩张等病理改变;与模型组比,三化汤高剂量组大鼠神经受损症状明显减轻(P<0.05),大鼠脑组织含水量明显降低(P<0.01)。结论:三化汤对大鼠脑缺血再灌注损伤具有一定的保护作用。
OBJECTIVE: To observe the effects of Sanhua Decoction on neurological function, brain water content, and pathological morphology of brain in rats with cerebral ischemia-reperfusion. METHODS: Rats were randomly divided into sham operation group, model group, low-dose high-dose group (7.2, 14.4 g.kg-1) ig, nimodipine group (8.1 mg.kg-) 1 ig, daily One time, continuous for 7 days, one week after administration, a rat model of cerebral ischemia-reperfusion was prepared by a suture method. After 2 hours of ischemia, 24 hours after reperfusion, Zealonga grade 5 method was used to score the symptoms of neurological damage. Brain wet weight and brain dry weight were weighed to calculate brain water content. HE staining was used to analyze brain pathological changes. RESULTS: Compared with the sham-operated group, the neurological deficits in the model group were severe (P<0.01), and the brain water content was significantly increased (P<0.01). The light nerve cells showed obvious swelling, degeneration, necrosis, and inflammatory cell infiltration. As compared with the model group, the symptoms of nerve damage in the high-dose group of Sanhua Decoction significantly decreased (P<0.05), and the brain tissue water content of the rats decreased significantly (P<0.01). Conclusion: Sanhua Decoction has a protective effect on cerebral ischemia-reperfusion injury in rats.