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背景:肾脏缺血再灌注常合并肾脏及肺脏的急性损伤,且角质细胞生长因子受体及钠通道蛋白在缺血再灌注致急性肾、肺损伤中的表达变化及α-促黑素的治疗作用有待于进一步观察及研究。目的:探索全肾缺血再灌注模型大鼠中角质细胞生长因子受体、钠通道蛋白的表达以及α-促黑素的治疗作用。方法:选择健康雄性SD大鼠30只按随机数字表法等分为对照组、缺血再灌注造模组和α-促黑素干预组。缺血再灌注造模组和α-促黑素组大鼠通过肾动脉结扎30 min建立全肾缺血/再灌注模型,对照组大鼠仅暴露肾动脉不结扎。α-促黑素干预组大鼠于造模前30 min腹腔注射α-促黑素(0.25 mg/kg)进行干预,缺血再灌注造模组大鼠注射等量(4 mL)生理盐水。结果与结论:与对照组相比,缺血再灌注造模组与α-促黑素干预组大鼠肾、肺组织含水量明显升高,角质细胞生长因子受体、钠通道蛋白的表达明显下降(P<0.05);与缺血再灌注造模组相比,α-促黑素干预组大鼠肾、肺组织含水量明显减少,而角质细胞生长因子受体、钠通道蛋白mR NA及蛋白的表达明显升高(P<0.05),且大鼠肾、肺组织充血水肿明显减轻。提示肾缺血再灌注后,角质细胞生长因子受体、钠通道蛋白的表达变化与肾、肺充血水肿等损伤一致,而α-促黑素可以增加角质细胞生长因子受体、钠通道蛋白的表达水平,减轻肾及肺组织的损伤,发挥一定的保护作用。
BACKGROUND: Renal ischemia / reperfusion is associated with acute kidney and lung injury, and the expression of keratinocyte growth factor receptor and sodium channel protein in acute kidney and lung injury induced by ischemia-reperfusion injury and the treatment of α-melanin The role needs to be further observed and studied. Objective: To explore the expression of keratinocyte growth factor receptor and sodium channel protein in rat model of global ischemia reperfusion and the therapeutic effect of a-melanin. Methods: Thirty healthy male SD rats were randomly divided into control group, ischemia-reperfusion model group and α-melanocyte intervention group according to random number table. Ischemia / reperfusion model group and a-melanocyte group rats were subjected to renal ischemia / reperfusion model by ligation of the renal artery for 30 min. Rats in the control group were exposed only to the renal artery without ligation. Rats in the α-melanocyte-stimulated group were injected with α-melanocyte (0.25 mg / kg) intraperitoneally 30 min before model establishment. Rats in the ischemia-reperfusion group were injected with the same amount of normal saline (4 mL). RESULTS AND CONCLUSION: Compared with the control group, the water content in the kidney and lung tissues of rats in the model group and the α-melanocyte-stimulated intervention group was significantly increased, and the expressions of keratinocyte growth factor receptor and sodium channel protein were significantly increased (P <0.05). Compared with ischemia / reperfusion model group, the water content of kidney and lung tissue in a-melanin intervention group decreased significantly, whereas keratinocyte growth factor receptor, sodium channel protein mR NA and Protein expression was significantly increased (P <0.05), and rat kidney and lung tissue congestion and edema significantly reduced. Prompted renal ischemia-reperfusion, keratinocyte growth factor receptor, sodium channel protein expression changes and renal, pulmonary congestion and edema and other injuries consistent, and alpha-melanin can increase the keratinocyte growth factor receptor, sodium channel protein The level of expression, reduce renal and lung injury, play a protective role.