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目的观察酪酸梭菌联合美沙拉嗪对小鼠溃疡性结肠炎(UC)的治疗作用,并探讨其可能的作用机制。方法用3%的葡聚糖硫酸钠(DSS)建立小鼠溃疡性结肠炎模型。50只BALB/c小鼠随机分为正常组、模型组、酪酸梭菌组、美沙拉嗪组、酪酸梭菌联合美沙拉嗪组。观察指标包括疾病活动指数(DAI)评分,结肠粘膜肉眼改变及病理组织学积分,采用免疫组化法检测结肠粘膜组织中HSP27的表达量,ELASA法检测血清中TNF-α的表达量。结果酪酸梭菌和美沙拉嗪都可以降低实验小鼠DAI积分,减轻肠道的组织学损伤程度;与模型组相比,酪酸梭菌组、美沙拉嗪组、酪酸梭菌联合美沙拉嗪组,结肠中HSP27的表达都上调(P<0.05),血清中TNF-α的表达都下降(P<0.05),其中美沙拉嗪组与酪酸梭菌组治疗效果相当(P>0.05),酪酸梭菌联合美沙拉嗪组治疗效果最佳(P<0.05)。结论酪酸梭菌和美沙拉嗪对急性期UC都有治疗作用,二者联合用药效果更佳,其部分机制可能与上调HSP27,抑制TNF-α的表达而减轻肠道的炎性反应有关。
Objective To observe the therapeutic effect of Clostridium butyricum and mesalazine on ulcerative colitis (UC) in mice and to explore its possible mechanism. Methods A mouse model of ulcerative colitis was established with 3% sodium dextran sulfate (DSS). Fifty BALB / c mice were randomly divided into normal group, model group, Clostridium butyricum group, mesalazine group, Clostridium butyricum group and mesalazine group. Observations included disease activity index (DAI) score, colorectal mucosal changes and histopathological scores. Immunohistochemistry was used to detect the expression of HSP27 in colonic mucosa tissues. ELASA method was used to detect the expression of TNF-α in serum. Results Both Clostridium butyricum and mesalazine could reduce the DAI score of experimental mice and alleviate the intestinal histological damage. Compared with the model group, Clostridium butyricum group, mesalazine group, Clostridium butyricum group and mesalazine group, The expression of HSP27 in colon was up-regulated (P <0.05), and the expression of TNF-α in serum was decreased (P <0.05). The mesalazine group and Clostridium butyricum group had the same treatment effect (P> 0.05) The combined mesalazine group was the best (P <0.05). Conclusions Clostridium butyricum and mesalazine have a therapeutic effect on UC in acute stage, and the combined effect of them is better. Some mechanisms may be related to up-regulating HSP27, inhibiting the expression of TNF-α and relieving the intestinal inflammatory reaction.