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植物组织中的葡萄糖在葡萄糖氧化酶 (GOD)作用下产生的过氧化氢可在辣根过氧化物酶 (HRP)的催化作用下使荧光素产生褪色反应 ,基于这一现象建立了一种选择性测定植物果实组织中葡萄糖含量的方法 .该方法可以根据检测对象的不同调整检测范围和灵敏度 ,同时具备了安全 ,方便的优点 .利用此法对苹果、鸭梨、酥梨中的葡萄糖含量进行了测定 ,结果与化学发光法一致
Hydrogen peroxide produced by glucose in plant tissues under the action of glucose oxidase (GOD) can produce a fading reaction of fluorescein under the action of horseradish peroxidase (HRP), establishing a choice based on this phenomenon Method for determining glucose content in fruit tissue of a plant The method can adjust the detection range and the sensitivity according to the detection object and has the advantages of safety and convenience at the same time ... ... The glucose content in the apple, the pear and the pear is measured by the method The determination, the results consistent with the chemiluminescence method