病毒性心肌炎患儿sIL-2R及抗ANT抗体的检测及意义

• 作 者: 李文强 文正万 邬松林 胡宝红 江钟炎
• 机 构: 湖北医科大学附属第一医院
• 出 处: 上海免疫学杂志
• 发表日期: 1999年01期
• 关键词: 心肌炎患儿 sIL-2R 抗体反应 病毒性心肌炎 心肌线粒体 体液免疫功能 疾病进程 患儿疾病 双抗体夹心 差异有显著性

用双抗体夹心ＥＬＩＳＡ法检测３８例病毒性心肌炎（ＶＭＣ）患儿及２８例正常儿的ｓＩＬ２Ｒ；另用免疫转印技术检测血清中抗犬心肌线粒体ＡＮＴ抗体。结果显示：（１）ＶＭＣ患儿ｓＩＬ２Ｒ明显高于正常组（Ｐ＜００１），ｓＩＬ２Ｒ水平与患儿射血分数（ＥＦ）及肌酸激酶（ＣＫＭＢ）之间有良好的相关性；（２）ＶＭＣ组血清抗犬ＡＮＴ抗体检出率为２６３％，正常组为０，差异有显著性意义（Ｐ＜００１）；（３）抗ＡＮＴ抗体主要出现在ｓＩＬ２Ｒ阳性组。提示ＶＭＣ患儿有细胞免疫及体液免疫功能紊乱，并在ＶＭＣ的发病及疾病进程中起重要作用；ｓＩＬ２Ｒ可作为反映ＶＭＣ患儿疾病活动情况及治疗、预后判断的指标；抗ＡＮＴ抗体检出说明体液免疫所致代谢紊乱也参与了ＶＭＣ的发病过程。

38 cases of viral myocarditis (VMC) and 28 normal children sIL 2R were detected by double antibody sandwich ELISA. Anti-canine myocardial mitochondrial ANT antibody was detected by immunoblotting in serum. The results showed that: (1) sIL2R in children with VMC was significantly higher than that in normal group (P <001), sIL2R level was positively correlated with EF and CKMB (2) The detection rate of anti-ANT antibody in VMC group was 263% and that in normal group was 0, the difference was significant (P <001); (3) Anti-ANT antibody mainly appeared In sIL 2R positive group. Suggesting that VMC children with cellular immunity and humoral immune dysfunction and VMC in the pathogenesis and progression of the disease plays an important role; sIL 2R can be used as a reflection of VMC disease activity and treatment, prognosis indicators; anti-ANT antibody test A note of metabolic disorders caused by humoral immunity is also involved in the pathogenesis of VMC.