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目的观察富含半胱氨酸的酸性分泌蛋白(SPARC)在ob/ob小鼠脂肪组织中的表达情况。方法选择10周龄的ob/ob小鼠(C57BL/6J-Lepob/J)与其同窝野生对照型小鼠各6只,取其腹部皮下脂肪组织,RT-PCR方法检测脂肪组织中 SPARC基因的表达,Western blot法测定SPARC目的蛋白的表达水平,免疫荧光染色法对脂肪组织进行染色观察。结果 SPARC在ob/ob小鼠脂肪组织中表达明显升高。结论 SPARC在ob/ob小鼠脂肪组织中呈现高表达,可能参与肥胖和糖尿病的发生发展。
Objective To observe the expression of cysteine-rich acidic secreted protein (SPARC) in ob / ob mouse adipose tissue. Methods Six obese ob / ob mice (C57BL / 6J-Lepob / J) and their wild-type control mice were selected and their abdominal subcutaneous adipose tissue was selected. RT-PCR was used to detect the expression of SPARC gene in adipose tissue The expression of SPARC protein was detected by Western blot and the adipose tissue was stained by immunofluorescence staining. Results SPARC expression was significantly increased in ob / ob mouse adipose tissue. Conclusion SPARC is highly expressed in adipose tissue of ob / ob mice and may be involved in the development of obesity and diabetes.