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目的探讨柳栎浸膏提取物对大鼠肾草酸钙结石形成的影响,并初步探讨其作用机制。方法采用乙二醇诱导大鼠肾草酸钙结石模型的建立,将60只Wistar大鼠随机分为对照组、模型组、柳栎浸膏低剂量组和柳栎浸膏高剂量组,每组15只,给药4周后,检测大鼠尿量、饮食量、体重,尿Ca2+、Mg2+、尿草酸和枸橼酸盐浓度,血肌酐(Cr)、尿素氮(BUN)和肾草酸浓度,肾脏中总超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性。结果与模型组比较,柳栎浸膏低剂量组能降低大鼠的饮水量和尿量,降低尿钙[(1.89±0.43)mmol/L vs(3.43±0.20)mmol/L]和尿草酸浓度[(2.02±0.02)mmol/L vs(3.83±1.04)mmol/L],提高枸橼酸盐[(9.03±1.02)mmol/L vs(8.24±0.39)mmol/L]和Mg2+浓度[(16.30±0.23)mmol/L vs(6.13±1.44)mmol/L],降低Cr、BUN和草酸含量,提高SOD[(230.19±20.69)U/mg vs(160.83±9.05)U/mg]和GSH-Px活性[(45.53±5.45)U/mg vs(37.13±3.44)U/mg(均P<0.05);与柳栎浸膏低剂量组比较,高剂量组上述结果改变更显著。结论柳栎浸膏能够增加尿中结石抑制物,减少结石形成促进因素,从而抑制草酸钙结石的形成。
Objective To investigate the effects of extract of Quercus acutissima on the formation of renal calcium oxalate stones in rats and its mechanism of action. Methods Sixty Wistar rats were randomly divided into control group, model group, low dose group of Willow oak extract and high dose of willow oak extract group, with 15 Only four weeks after administration, the urine output, diet, body weight, urinary Ca2 +, Mg2 +, urinary oxalate and citrate concentrations, serum creatinine (Cr), blood urea nitrogen (BUN) Total superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity. Results Compared with the model group, the low dosage of Quercus acutissima extract could reduce the water intake and urine volume of rats and decrease the levels of urinary calcium [(1.89 ± 0.43) mmol / L vs (3.43 ± 0.20) mmol / L] and urinary oxalate ([(2.02 ± 0.02) mmol / L vs (3.83 ± 1.04) mmol / L] and citrate ± 0.23 mmol / L vs 6.13 ± 1.44 mmol / L], and decreased the content of Cr, BUN and oxalic acid, and increased the level of SOD [(230.19 ± 20.69) U / mg vs (160.83 ± 9.05) U / mg) and GSH-Px (45.53 ± 5.45) U / mg vs (37.13 ± 3.44) U / mg respectively (all P <0.05). Compared with the low dose group of the oak oak extract, the above-mentioned results of the high dose group changed more significantly. Conclusion Willow oak extract can increase urinary stone inhibitors, reducing the formation of stone promoting factors, thereby inhibiting the formation of calcium oxalate stones.