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p53基因是与人类肿瘤相关性最高的抑癌基因 ,它多方面的功能主要是通过激活或抑制大量p53下游基因的表达来完成的。目前主要使用两种策略鉴定p53下游基因 :一种是消减杂交和差异扫描 ;另一种是利用p53下游基因在启动子区域或者内含子内包含有p53结合位点。已经鉴定的p53下游基因主要包括p53负调控基因mdm2 ;细胞周期负调控相关基因p2 1waf 1/cip 1,14 3 3 σ ,cyclinG ;DNA复制与修复基因gadd 45;细胞永生化基因igf bp3 ;细胞凋亡相关基因bax ,gml,p2xm ,killer/dr 5和pag 60 8;抑制血管生成基因tsp 1;以及应答细胞胁迫基因tp 53tg1,csr和pig 3等
The p53 gene is the most tumor-suppressor gene associated with human tumors. Its multiple functions are mainly through the activation or inhibition of the expression of a large number of downstream genes of p53. Currently, two strategies are mainly used to identify the downstream genes of p53: one is subtractive hybridization and differential scanning; the other is the use of p53 downstream genes to include p53 binding sites in the promoter region or intron. Down-regulated p53 genes include mdm2, a negative regulator of p53, p21waf1 / cip1,143-3a, and cyclinG; gadd45, an immortalized gene igf bp3; Apoptosis-related genes bax, gml, p2xm, killer / dr 5 and pag 60 8; inhibition of angiogenesis gene tsp 1; and repression of cell stress genes tp 53tg1, csr and pig 3