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【目的】探究甲酸对木糖乙醇发酵模式菌株休哈塔假丝酵母基因转录的影响规律,发掘抑制的目标基因及抑制特征。【方法】基于该酵母葡萄糖、木糖代谢转录组的研究成果,结合细胞代谢途径和人工基因比对筛选出乙醇发酵相关基因,再通过RQ-PCR对梯度甲酸抑制条件下的上述基因进行定量分析,进而发掘出甲酸抑制的目标基因。【结果】共筛选出42个相关基因,经RQ-PCR定量分析最终确定受甲酸显著抑制的基因10个,上调基因5个。【结论】在基因转录水平上,休哈塔假丝酵母中甲酸显著抑制的基因按照抑制强度降序排列为XYL2、ACS、RKI、TAL、GND1、TKL、ZWF1、XYL1、PDH和PDC,按照上调强度降序排列为ALD、GLK、MDH、PFK、ADH。
【Objective】 The purpose of this study was to investigate the effect of formic acid on the gene transcription of Candida shehatae in xylose ethanol fermentation model and explore the target genes and their inhibitory characteristics. 【Method】 Based on the research results of yeast glucose and xylose metabolism transcriptome, the genes related to ethanol fermentation were screened by combining cell metabolic pathway and artificial gene alignment. The above genes were further analyzed by RQ-PCR under the condition of gradient formic acid inhibition , And then discover the target gene inhibited by formic acid. 【Result】 Totally 42 related genes were screened out and 10 genes significantly inhibited by formic acid were identified by RQ-PCR. Five genes were up-regulated. 【Conclusion】 Genes that were significantly inhibited by Candida albicans in Candida shehatae were arranged in descending order of XYL2, ACS, RKI, TAL, GND1, TKL, ZWF1, XYL1, PDH and PDC at gene transcription level, Descending order of ALD, GLK, MDH, PFK, ADH.