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目的:观察百诃清金颗粒对气道高反应性大鼠气道炎症的干预作用。方法:将48只SD大鼠随机分为空白组、模型组、地塞米松组、百诃清金颗粒低、中、高剂量组,每组8只;采用卵白蛋白致敏,复制气道高反应性大鼠模型。按实验计划干预后,记录各组支气管肺泡灌洗液(BALF)中白细胞总数及分类计数;检测白介素-4(IL-4)、白介素-6(IL-6)、干扰素-γ(IFN-γ)、肿瘤坏死因子(TNF-α)的含量;观察肺组织HE染色病理学特点。结果:百诃清金颗粒高、中剂量组可明显降低气道高反应大鼠BALF中白细胞、嗜酸性粒细胞(P<0.001)及中性粒细胞数量(P<0.05),并减轻肺组织炎症损伤。与空白组相比,模型组IL-4、IL-6、TNF-α水平显著升高(P<0.001),IFN-γ水平显著下降(P<0.001)。高剂量组可显著降低IL-4(P<0.001)、IL-6、TNF-α(P<0.01)表达,升高IFN-γ(P<0.01)水平,效果与地米组相当;中、低剂量组IL-4、TNF-α水平较虽明显低于模型组,但差异无统计学意义。结论:百诃清金颗粒可抑制气道高反应性大鼠的气道炎症反应,降低气道高反应性,且呈明显量效关系;这可能是其治疗感染后咳嗽等以气道反应性增高为病理表现疾病的部分作用机制。
Objective: To observe the intervention effects of Baihaoc Qingjin Granules on airway inflammation in rats with airway hyperresponsiveness. Methods: 48 Sprague-Dawley rats were randomly divided into blank group, model group, dexamethasone group, and Baihacao Qingjin Granules in low, medium and high dose groups, with 8 rats in each group. Alveolar epithelial cells were sensitized and overexpressed Reactive rat model. After intervention according to the experimental plan, the total number of leukocytes in the bronchoalveolar lavage fluid (BALF) of each group and the classification and counting were recorded. The levels of interleukin-4, interleukin-6, IFN- γ) and tumor necrosis factor (TNF-α) were detected. The pathological features of HE staining were observed. Results: High and medium doses of Baihacaoqingjin Granules could significantly reduce the number of leukocytes, eosinophils (P <0.001) and the number of neutrophils (P <0.05) in BALF of rats with airway hyperresponsiveness, Inflammatory damage. Compared with the blank group, the levels of IL-4, IL-6 and TNF-α in the model group were significantly increased (P <0.001) and IFN-γ levels were significantly decreased (P <0.001). High dose group could significantly reduce the expression of IL-4 (P <0.001), IL-6 and TNF-α (P <0.01) Low-dose group IL-4, TNF-α levels were significantly lower than the model group, but no significant difference. Conclusions: Baihaochajinjin Granule can inhibit the airway inflammatory response and reduce the airway hyperresponsiveness in the airway hyperresponsiveness rats, and there is a significant dose-response relationship. This may be due to the airway reactivity Elevated pathological manifestations of the disease part of the mechanism of action.