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目的:从肝癌及门静脉癌栓中分别取材建立人肝癌细胞系,探讨其生物学特性。方法:取人肝癌及门静脉癌栓新鲜手术标本,利用胶原酶消化法进行肿瘤细胞原代培养并扩大克隆培养建系。采用光镜、电镜、染色体核型分析及异种移植瘤实验对新建细胞系生物学特性进行观察。结果:来源于门静脉癌栓的人肝癌细胞在体外稳定培养已经将近1年,传至100余代,命名为CSQT-1。该细胞系具有典型的恶性上皮细胞特征,其群体倍增时间为48h;染色体中位数为87～90,为亚四倍体;裸鼠皮下异种移植可形成移植瘤,该细胞系中CD133+表达比较稳定。结论:细胞系特征显示该细胞系是一株新建的来源于门静脉癌栓的人肝癌细胞系。
Objective: To investigate the biological characteristics of human hepatocellular carcinoma cell lines from hepatocellular carcinoma and portal vein tumor thrombus respectively. METHODS: Fresh surgical specimens of human hepatocellular carcinoma and portal vein tumor thrombus were taken. The primary culture of tumor cells was performed by collagenase digestion and the clonal culture line was expanded. The biological characteristics of the newly established cell lines were observed by light microscopy, electron microscopy, chromosome karyotyping and xenograft tumor experiments. RESULTS: Human hepatocellular carcinoma cells derived from portal vein tumor thrombi were stable cultured in vitro for nearly one year and passed to more than 100 generations and named as CSQT-1. The cell line has typical characteristics of malignant epithelial cells, the population doubling time is 48h, the median of chromosomes is 87-90, sub-tetraploid; xenotransplantation of nude mice can form xenografts, the expression of CD133 + in this cell line stable. Conclusion: The characteristics of cell lines show that this cell line is a newly constructed human hepatoma cell line derived from portal vein tumor thrombus.