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目的探讨哮喘患者体内IL-35与CD8+T细胞之间的关系及IL-35在哮喘患者体内可能的作用。方法采集哮喘患者和正常人外周静脉血,分离、培养外周血单个核细胞(PBMCs);流式细胞术检测Tregs、Tc1、Tc2和Tc17细胞比例;ELISA检测血浆IL-35、IFN-γ、IL-4和IL-17水平。采用Pearson检验分析哮喘患者血浆IL-35与Tc1、Tc2和Tc17细胞及其相关细胞因子之间的关系。结果与正常人相比,哮喘患者外周血Tregs细胞及IL-35表达水平明显下降(P<0.01);Tc2和Tc17细胞及相关细胞因子(IL-4、IL-17)显著增高(P<0.05);血浆IL-35水平与外周血Tc2细胞数目及IL-4水平成明显负相关性(P<0.01)。结论哮喘患者血浆IL-35与外周血分泌IL-4的CD8+T(Tc2)细胞成负相关,体内降低的IL-35可能参与了哮喘患者的发病过程。
Objective To investigate the relationship between IL-35 and CD8 + T cells in asthmatic patients and the possible role of IL-35 in asthmatic patients. Methods Peripheral venous blood was collected from patients with asthma and normal controls, peripheral blood mononuclear cells (PBMCs) were isolated and cultured. The proportion of Tregs, Tc1, Tc2 and Tc17 were detected by flow cytometry. The levels of IL-35, IFN- -4 and IL-17 levels. Pearson test was used to analyze the relationship between plasma IL-35 and Tc1, Tc2 and Tc17 cells and related cytokines in asthmatic patients. Results Compared with normal controls, the expression of Tregs and IL-35 in peripheral blood of asthmatic patients was significantly decreased (P <0.01), while the levels of Tc2 and Tc17 and related cytokines (IL-4 and IL-17) ). The level of plasma IL-35 was negatively correlated with the number of Tc2 cells and the level of IL-4 in peripheral blood (P <0.01). Conclusion IL-35 in plasma of patients with asthma is negatively correlated with CD8 + T (Tc2) cells secreting IL-4 in peripheral blood. IL-35 in vivo may be involved in the pathogenesis of asthma.