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ELISA已用于检测各类标本中的微生物抗原,但存存问题是某些标本的本底过高,敏感性有限,标本中微生物对健康有害等。本文研究用SDS灭活作为抗原的环状病毒,提高了ELISA的敏感性。 2株环状病毒(GI病毒和CW病毒)用BHK-21细胞培养,用空斑形成技术检查其传染性。制备多克隆超免疫小鼠腹腔液。细胞收获液中加入含20%SDS的PBS,使依次含有0.1、0.5或1.0%SDS,37℃2小时或100℃5分钟。用PBS对倍稀释,使稀释度为1∶25～1∶1600,在直接ELISA中用以包被半板。取96孔聚苯乙烯
ELISA has been used to detect various types of specimens of microbial antigens, but the storage problem is the background of some specimens is too high, the sensitivity is limited, the samples of microorganisms harmful to health and so on. In this paper, the use of SDS inactivation as an antigen of the circular virus, increased ELISA sensitivity. Two strains of circulating viruses (GI and CW viruses) were cultured in BHK-21 cells and their infectivity was examined by plaque-forming technique. Preparation of polyclonal hyperimmune mouse peritoneal fluid. The cells were harvested by adding 20% SDS in PBS containing 0.1, 0.5 or 1.0% SDS, followed by 37 ° C for 2 hours or 100 ° C for 5 minutes. Dilute with PBS to a dilution of 1:25 to 1: 1600 and coat the half-plate in a direct ELISA. Take 96-well polystyrene