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复制动物肝脏肿瘤可采用诱发性或移植性方法。移植性肿瘤模型试验周期一般较短，易于控制而多被采用。杨继金[1]等报道用Walker－256瘤株进行肝包膜下包埋瘤块制作转移性肝癌模型，操作相对复杂，不能形成弥散型肝癌模型。作者利用Walker256瘤株接种腹腔形成腹水细胞、冻存Walk
Replication of animal liver tumors can be induced or transplanted. The experimental cycle of transplantable tumor models is generally short, easy to control and often used. Yang Jijin et al. [1] reported that the use of Walker-256 tumor-bearing subcutaneously coated tumor masses to make metastatic liver cancer models is relatively complicated and can not form a diffuse liver cancer model. The authors used the Walker256 tumor strain to inoculate the abdominal cavity to form ascites cells and cryopreserved Walk